{
  "abstract": "Transcriptional condensates are clusters of transcription factors, coactivators, and RNA Pol II associated with gene activation, yet how they assemble and function within the cell remains unclear. Here, we show that transcriptional condensates form in a stepwise manner to enable both graded and three-dimensional (3D) gene control in the yeast heat shock response (HSR). First, the transcription factor Hsf1 (heat shock factor 1) clusters upon partial dissociation from the chaperone Hsp70. Next, the coactivator Mediator partitions following further Hsp70 dissociation and Hsf1 phosphorylation. Finally, Pol II condenses, driving emergent coalescence of HSR genes. Separation-of-function Hsf1 mutants revealed graded (non-switch-like) control of transcription and a decoupling of condensate formation and gene activation. Fully assembled HSR condensates promoted adaptive 3D genome reconfiguration, suggesting a role beyond transcription. In the HSR, differential condensation of the transcriptional machinery quantitatively tunes gene expression and qualitatively remodels the 3D genome.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL, USA. Electronic address: schowdhary@uchicago.edu",
    "Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL, USA",
    "Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL, USA; Institute for Biophysical Dynamics, University of Chicago, Chicago, IL, USA; Center for Physics of Evolution, University of Chicago, Chicago, IL, USA. Electronic address: pincus@uchicago.edu"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Surabhi Chowdhary",
      "orcid": "0000-0002-7801-2525"
    },
    {
      "affs": [
        1
      ],
      "name": "Sarah Paracha",
      "orcid": "0000-0002-4047-218X"
    },
    {
      "affs": [
        1
      ],
      "name": "Lucas Dyer"
    },
    {
      "affs": [
        2
      ],
      "name": "David Pincus",
      "orcid": "0000-0002-9651-6858"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6sdy6s7xpqpmc.bib",
  "cited_by": 10,
  "doi": "10.1016/j.celrep.2026.117888",
  "fulltext_url": null,
  "id": "6sdy6s7xpqpmc",
  "issns": [
    "2211-1247",
    "2639-1856"
  ],
  "landing_url": "https://doi.org/10.1016/j.celrep.2026.117888",
  "language": "en",
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Genomics and Chromatin Dynamics"
    ]
  ],
  "openalex": "W7204003684",
  "pdf_url": null,
  "pmcid": "PMC11888319",
  "pmid": "40060634",
  "published": "2026-08-22",
  "publisher": "Cell Press",
  "ris": "https://research.draconitic.com/paper/6sdy6s7xpqpmc.ris",
  "sources": [
    {
      "first_seen": "2026-11-05T13:20:47Z",
      "native_id": "W7204003684",
      "source": "openalex",
      "url": "https://doi.org/10.1016/j.celrep.2026.117888"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Genomics and Chromatin Dynamics",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Emergent 3D genome reorganization and graded gene control from the stepwise assembly of transcriptional condensates",
  "title_text": "Emergent 3D genome reorganization and graded gene control from the stepwise assembly of transcriptional condensates",
  "type": "peer-reviewed",
  "updated_at": "2026-11-07T13:20:49Z",
  "url": "https://research.draconitic.com/paper/6sdy6s7xpqpmc",
  "venue": "Cell Reports",
  "versions": []
}