{
  "abstract": "The nitrogenase-like reductase CfbC/D catalyzes the six-electron reduction of the tetrapyrrole macrocycle of Ni2+-sirohydrochlorin a,c-diamide during the biosynthesis of coenzyme F430, an essential cofactor for biological methane formation and consumption. The individual dimeric protein components, CfbC2 and CfbD2, form a complex to allow for electron transfer from the [4Fe:4S] cluster in CfbC2 to the [4Fe:4S] cluster in CfbD2 and onto the substrate. The overall process is ATP-dependent. Although CfbC/D belongs to the family of nitrogenase-like enzymes, there are several intriguing differences between CfbC/D, other nitrogenase-like enzymes and nitrogenase itself. In contrast to the other enzymes, CfbC/D exhibits a simpler molecular architecture, forming a heterotetrameric CfbC2D2 assembly. Furthermore, the interaction of the CfbC and CfbD components does not depend on the presence of adenosine triphosphate (ATP). Here we report cryoelectron microscopy structures of the CfbC/D complex prepared in the absence and presence of substrates. These structures not only shed light on the molecular architecture of the CfbC/D complex but also reveal a high degree of structural flexibility within the CfbD2 component, which undergoes a large conformational change in the presence of an ATP analog and the tetrapyrrole substrate. The interplay of CfbD with its reductase CfbC also strongly suggests an evolutionary pathway toward the modern nitrogenase from methanogenic archaea.",
  "abstract_shown": true,
  "affiliations": [
    "Institut für Biochemie, Fakultät für Chemie und Pharmazie, Albert-Ludwigs-Universität Freiburg, Freiburg 79104, Germany",
    "Faculty of Synthetic Biology and Institute of Emerging Agricultural Technology, Shenzhen University of Advanced Technology, Shenzhen 518107, China",
    "Institut für Pharmazeutische Wissenschaften, Fakultät für Chemie und Pharmazie, Albert-Ludwigs-Universität Freiburg, Freiburg 79104, Germany",
    "Centre for Integrative Biological Signalling Studies, University of Freiburg, Freiburg 79104, Germany"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Lin Zhang",
      "orcid": "0000-0001-5431-5006"
    },
    {
      "affs": [
        2
      ],
      "name": "Florian Schuhmacher",
      "orcid": "0009-0002-7696-1193"
    },
    {
      "affs": [
        2
      ],
      "name": "Alena Kussler"
    },
    {
      "affs": [
        0,
        3
      ],
      "name": "Oliver Einsle",
      "orcid": "0000-0001-8722-2893"
    },
    {
      "affs": [
        2
      ],
      "name": "Gunhild Layer",
      "orcid": "0000-0003-0974-173X"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6t33658edq878.bib",
  "cited_by": 0,
  "doi": "10.1073/pnas.2611794123",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13715749/",
  "id": "6t33658edq878",
  "issns": [
    "0027-8424",
    "1091-6490"
  ],
  "landing_url": "https://doi.org/10.1073/pnas.2611794123",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "407"
    ],
    [
      "heading",
      "Biochemistry"
    ],
    [
      "heading",
      "Biological Sciences"
    ],
    [
      "openalex",
      "2105|Metalloenzymes and iron-sulfur proteins"
    ]
  ],
  "openalex": "W7223001638",
  "pdf_url": null,
  "pmcid": "PMC13715749",
  "pmid": "42900713",
  "published": "2026-10-20",
  "publisher": "National Academy of Sciences",
  "ris": "https://research.draconitic.com/paper/6t33658edq878.ris",
  "sources": [
    {
      "first_seen": "2026-11-01T12:22:57Z",
      "native_id": "PMC13715749",
      "source": "pmc",
      "url": "https://doi.org/10.1073/pnas.2611794123"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "journal-openalex:1312|Genomics and Chromatin Dynamics",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Conformational flexibility of the nitrogenase-like reductase CfbC/D involved in coenzyme F430 biosynthesis",
  "title_text": "Conformational flexibility of the nitrogenase-like reductase CfbC/D involved in coenzyme F430 biosynthesis",
  "type": "peer-reviewed",
  "updated_at": "2026-11-01T12:22:57Z",
  "url": "https://research.draconitic.com/paper/6t33658edq878",
  "venue": "Proceedings of the National Academy of Sciences of the United States of America",
  "versions": []
}