{
  "abstract": "To understand gene function, the encoding DNA or mRNA transcript can be manipulated and the consequences observed. However, these approaches do not have a direct effect on the protein product of the gene, which is either permanently abrogated or depleted at a rate defined by the half-life of the protein. We therefore developed a single-component system that could induce the rapid degradation of the specific endogenous protein itself. A construct combining the RING domain of ubiquitin E3 ligase RNF4 with a protein-specific camelid nanobody mediates target destruction by the ubiquitin proteasome system, a process we describe as antibody RING-mediated destruction (ARMeD). The technique is highly specific because we observed no off-target protein destruction. Furthermore, bacterially produced nanobody-RING fusion proteins electroporated into cells induce degradation of target within minutes. With increasing availability of protein-specific nanobodies, this method will allow rapid and specific degradation of a wide range of endogenous proteins.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "name": "Adel F.M. Ibrahim"
    },
    {
      "name": "Linnan Shen"
    },
    {
      "name": "Michael Tatham"
    },
    {
      "name": "David Dickerson"
    },
    {
      "name": "Alan Prescott"
    },
    {
      "name": "Naima Abidi"
    },
    {
      "name": "Dimitris Xirodimas"
    },
    {
      "name": "Ronald Hay"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6wxhd4t16aq99.bib",
  "cited_by": 64,
  "doi": "10.1016/j.molcel.2020.04.032",
  "fulltext_url": null,
  "id": "6wxhd4t16aq99",
  "issns": [
    "1097-2765",
    "1097-4164"
  ],
  "landing_url": "https://hal.science/hal-05751779v1",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "openalex",
      "1312|Ubiquitin and proteasome pathways"
    ]
  ],
  "openalex": "W3027477830",
  "pdf_url": "http://www.cell.com/article/S1097276520302744/pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-16",
  "publisher": "Cell Press",
  "ris": "https://research.draconitic.com/paper/6wxhd4t16aq99.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:35Z",
      "native_id": "hal-05751779",
      "source": "hal",
      "url": "https://hal.science/hal-05751779v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Ubiquitin and proteasome pathways",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Antibody RING-Mediated Destruction of Endogenous Proteins",
  "title_text": "Antibody RING-Mediated Destruction of Endogenous Proteins",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:35Z",
  "url": "https://research.draconitic.com/paper/6wxhd4t16aq99",
  "venue": "Molecular Cell",
  "versions": []
}