{
  "abstract": "ObjectiveCerebral ischemia–reperfusion (I/R) injury is a major contributor to neuronal damage and long-term neurological dysfunction after stroke. Our previous studies demonstrated that miR-140-5p expression is markedly upregulated following cerebral ischemia and that in vivo overexpression of miR-140-5p exacerbates cognitive impairment, suppresses neurogenesis, and promotes neuronal death in ischemic mouse models; however, its molecular mechanisms remain unclear. This study aimed to investigate the downstream molecular targets of miR-140-5p and to elucidate the mechanisms by which miR-140-5p regulates neuronal injury after I/R.MethodsWe established an oxygen–glucose deprivation/reoxygenation (OGD/R) model in primary cortical neurons and assessed viability via Cell Counting Kit-8 (CCK-8). miR-140-5p expression after OGD/R was examined, and loss-of-function experiments evaluated the effects of miR-140-5p inhibition. Synaptic integrity was assessed by immunofluorescence, and inflammatory cytokines by enzyme-linked immunosorbent assay (ELISA). Proteomic analysis identified potential downstream targets, which were validated via Western blotting and dual-luciferase reporter assays.ResultsWe found that miR-140-5p expression was significantly upregulated in mouse cortical neurons after OGD/R. Functional inhibition of miR-140-5p markedly enhanced neuronal survival, promoted synaptic remodeling, and attenuated inflammatory responses following OGD/R injury. Mechanistically, miR-140-5p was shown to directly target Il1rapl1 and modulate downstream c-Jun N-terminal kinase (JNK) signaling, thereby regulating neuronal viability, inflammation, and synaptic plasticity under ischemia–reperfusion conditions.ConclusionThis study reveals a novel miR-140-5p–mediated regulatory mechanism in neuronal injury following cerebral ischemia–reperfusion and suggests a potential therapeutic target for ischemic brain injury.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Neurology and Institute of Neurology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China",
    "Department of Geriatric Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Yuan-yue Song"
    },
    {
      "affs": [
        1
      ],
      "name": "Yuan-yue Song"
    },
    {
      "affs": [
        0
      ],
      "name": "Zhu-jun Chen"
    },
    {
      "affs": [
        0
      ],
      "name": "Heng-yu Yan"
    },
    {
      "affs": [
        0
      ],
      "name": "Li-li Zeng"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6wzvpn6g5psar.bib",
  "cited_by": 0,
  "doi": "10.3389/fnmol.2026.1972044",
  "fulltext_url": "https://www.frontiersin.org/articles/10.3389/fnmol.2026.1972044/full",
  "id": "6wzvpn6g5psar",
  "issns": [
    "1662-5099"
  ],
  "landing_url": "https://doi.org/10.3389/fnmol.2026.1972044",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "RC321-571"
    ],
    [
      "openalex",
      "1306|MicroRNA in disease regulation"
    ]
  ],
  "openalex": "W7223326934",
  "pdf_url": "https://www.frontiersin.org/journals/molecular-neuroscience/articles/10.3389/fnmol.2026.1972044/pdf",
  "pmcid": "PMC13739790",
  "pmid": "42996358",
  "published": "2026-10-23",
  "publisher": "Frontiers Media S.A.",
  "ris": "https://research.draconitic.com/paper/6wzvpn6g5psar.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:43:48Z",
      "native_id": "a43c66d0f1724e6e9600e759abaf24cb",
      "source": "doaj",
      "url": "https://doi.org/10.3389/fnmol.2026.1972044"
    },
    {
      "first_seen": "2026-11-09T15:20:34Z",
      "native_id": "MED:42996358",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42996358"
    }
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  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:1306|MicroRNA in disease regulation",
      "subfield": "oncology"
    }
  ],
  "title": "Inhibition of miR-140-5p attenuates OGD/R-induced neuronal injury by targeting IL1RAPL1",
  "title_text": "Inhibition of miR-140-5p attenuates OGD/R-induced neuronal injury by targeting IL1RAPL1",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T15:20:34Z",
  "url": "https://research.draconitic.com/paper/6wzvpn6g5psar",
  "venue": "Frontiers in Molecular Neuroscience",
  "versions": []
}