{
  "abstract": "Here, we present a confocal microscopy protocol to determine the localization of Mycobacterium tuberculosis chorismate mutase (MtbCM) in trans-Golgi compartment. We co-transfect HEK293 cells with either pEGFP-C1-MtbCM and mCherry-TGOLN2 (trans-Golgi marker) or pEGFP-C1 and mCherry-TGOLN2 as a control, using Lipofectamine 2000. We describe steps for constructing pEGFP-C1-MtbCM and performing co-transfection and image acquisition using confocal microscopy. This protocol provides a representative procedure to determine the subcellular localization of the mycobacterial protein to the trans-Golgi compartment. For complete details on the use and execution of this protocol, please refer to Pal et al.¹.",
  "abstract_shown": true,
  "affiliations": [
    "Laboratory of Molecular Cell Biology, BRIC-Centre for DNA Fingerprinting and Diagnostics (CDFD), Inner Ring Road, Uppal, Hyderabad 500039, Telangana, India; Graduate Studies, Regional Centre for Biotechnology, Faridabad 121001, Haryana (NCR Delhi), India.",
    "Laboratory of Molecular Cell Biology, BRIC-Centre for DNA Fingerprinting and Diagnostics (CDFD), Inner Ring Road, Uppal, Hyderabad 500039, Telangana, India. Electronic address: sangitacdfd@gmail.com."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 2,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Vandana Maurya"
    },
    {
      "affs": [
        1
      ],
      "name": "Sangita Mukhopadhyay"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6xdez6jep7cyr.bib",
  "cited_by": 0,
  "doi": "10.1016/j.xpro.2026.104859",
  "fulltext_url": "https://europepmc.org/articles/PMC13672857",
  "id": "6xdez6jep7cyr",
  "issns": [
    "2666-1667"
  ],
  "landing_url": "https://europepmc.org/article/MED/42837261",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2725|Tuberculosis Research and Epidemiology"
    ]
  ],
  "openalex": "W7220455914",
  "pdf_url": "https://europepmc.org/articles/PMC13672857?pdf=render",
  "pmcid": "PMC13672857",
  "pmid": "42837261",
  "published": "2026-10-05",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/6xdez6jep7cyr.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:10Z",
      "native_id": "MED:42837261",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42837261"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2725|Tuberculosis Research and Epidemiology",
      "subfield": "infectious-disease"
    }
  ],
  "title": "Protocol for confocal microscopy-based localization of chorismate mutase protein of Mycobacterium tuberculosis in trans-Golgi compartment.",
  "title_text": "Protocol for confocal microscopy-based localization of chorismate mutase protein of Mycobacterium tuberculosis in trans-Golgi compartment.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:10Z",
  "url": "https://research.draconitic.com/paper/6xdez6jep7cyr",
  "venue": "STAR protocols",
  "versions": []
}