{
  "abstract": "Condensates at synapses organize synaptic vesicle (SV) clusters and are essential for efficient neurotransmitter release. While it is established that RNA granules traffic along axons, the function of RNA at the presynapse remains unclear. Here, we uncover a direct structural role of coding RNAs in organizing presynaptic condensates by focusing on SV clusters, condensates between synapsin‐1 and lipid vesicles. Using in vitro reconstitution systems, we show that RNA drives synapsin‐1 coacervation, with structured RNAs being more effective at promoting phase transitions. The importance of RNA was confirmed in living synapses, where acute disruption of native RNA induces a dispersion of SVs and synapsin. Conversely, ectopically expressed SV‐like condensates contained the translational machinery. The microscopy‐based in vitro translation assay demonstrates increased translation efficiency within synapsin‐1/RNA condensates. Together, our work indicates a novel structural role of RNAs in modulating SV condensates.",
  "abstract_shown": true,
  "affiliations": [
    "Whitman Center Marine Biological Laboratory Woods Hole Massachusetts USA",
    "Institute of Biochemistry Charité‐Universitätsmedizin Berlin Corporate Member of Freie Universität Berlin Humboldt‐Universität Berlin and Berlin Institute of Health & Laboratory of Molecular Neuroscience German Center for Neurodegenerative Diseases (DZNE) Berlin Germany",
    "Institute of Biology Freie Universität Berlin Berlin Germany",
    "Department of Cell Biology Duke University Durham North Carolina USA",
    "Human Biology and Primate Evolution Freie Universität Berlin Berlin Germany",
    "Max Planck Institute for Molecular Cell Biology and Genetics Dresden Germany",
    "Department of Molecular Immunology Groningen Biomolecular Sciences and Biotechnology Institute University of Groningen Groningen The Netherlands",
    "Department of Neuropathology Charité ‐ Universitätsmedizin Berlin Corporate Member of Freie Universität Berlin Humboldt‐Universität Zu Berlin and Berlin Institute of Health & German Center for Neurodegenerative Diseases (DZNE) Berlin Germany",
    "Goethe University Frankfurt Institute For Molecular Biosciences Frankfurt am Main Germany",
    "Institute For Neuro‐ and Sensory Physiology University Medical Center Göttingen Göttingen Germany",
    "Einstein Center for Neuroscience Charité‐Universitätsmedizin Berlin Corporate Member of Freie Universität Berlin Humboldt‐Universität Berlin and Berlin Institute of Health Berlin Germany"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 22,
  "authors": [
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Branislava Rankovic",
      "orcid": "0000-0001-8642-9809"
    },
    {
      "affs": [
        0,
        3
      ],
      "name": "Zachary M. Geisterfer",
      "orcid": "0000-0001-5883-6036"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Akshita Chhabra",
      "orcid": "0000-0002-7323-4989"
    },
    {
      "affs": [
        4
      ],
      "name": "Vladimir M. Jovanovic",
      "orcid": "0000-0002-8056-5065"
    },
    {
      "affs": [
        3,
        5
      ],
      "name": "Ian Seim",
      "orcid": "0000-0001-9719-2552"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Christian Hoffmann",
      "orcid": "0000-0003-3478-2410"
    },
    {
      "affs": [
        1
      ],
      "name": "Antonela Condric",
      "orcid": "0009-0006-5898-848X"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Gerard Aguilar Pérez",
      "orcid": "0009-0004-4995-1939"
    },
    {
      "affs": [
        6
      ],
      "name": "Rinse de Boer",
      "orcid": "0000-0002-5350-2683"
    },
    {
      "affs": [
        6
      ],
      "name": "Lars T. Hofstede",
      "orcid": "0009-0006-1138-0151"
    },
    {
      "affs": [
        1
      ],
      "name": "Jingao Sun",
      "orcid": "0009-0004-5395-1499"
    },
    {
      "affs": [
        7
      ],
      "name": "Kiara Freitag",
      "orcid": "0000-0002-5244-3140"
    },
    {
      "affs": [
        4
      ],
      "name": "Katja Nowick",
      "orcid": "0000-0003-3993-4479"
    },
    {
      "affs": [
        7
      ],
      "name": "Marina Jendrach",
      "orcid": "0000-0002-8601-2277"
    },
    {
      "affs": [
        7
      ],
      "name": "Frank L. Heppner",
      "orcid": "0000-0001-9816-8917"
    },
    {
      "affs": [
        8
      ],
      "name": "Vladimir Despic"
    },
    {
      "affs": [
        8
      ],
      "name": "Michaela Müller‐McNicoll",
      "orcid": "0000-0002-7174-8310"
    },
    {
      "affs": [
        2
      ],
      "name": "Stephan Sigrist",
      "orcid": "0000-0002-1698-5815"
    },
    {
      "affs": [
        6
      ],
      "name": "Geert van den Bogaart",
      "orcid": "0000-0003-2180-6735"
    },
    {
      "affs": [
        9
      ],
      "name": "Sofiia Reshetniak",
      "orcid": "0000-0003-4847-4144"
    },
    {
      "affs": [
        1,
        3
      ],
      "name": "Amy S. Gladfelter",
      "orcid": "0000-0002-2490-6945"
    },
    {
      "affs": [
        0,
        1,
        10
      ],
      "name": "Dragomir Milovanovic",
      "orcid": "0000-0002-6625-1879"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6xjn32wc4qhqh.bib",
  "cited_by": 0,
  "doi": "10.1002/advs.77115",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13496291/",
  "id": "6xjn32wc4qhqh",
  "issns": [
    "2198-3844"
  ],
  "landing_url": "https://doi.org/10.1002/advs.77115",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research Article"
    ],
    [
      "openalex",
      "1312|RNA Research and Splicing"
    ]
  ],
  "openalex": "W7203885131",
  "pdf_url": null,
  "pmcid": "PMC13496291",
  "pmid": "42627687",
  "published": "2026-08-21",
  "publisher": "Wiley",
  "ris": "https://research.draconitic.com/paper/6xjn32wc4qhqh.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T12:23:44Z",
      "native_id": "PMC13496291",
      "source": "pmc",
      "url": "https://doi.org/10.1002/advs.77115"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|RNA Research and Splicing",
      "subfield": "molecular-biology"
    }
  ],
  "title": "RNA Promotes Synapsin Condensates That Organize Synaptic Vesicles in Live Neurons and Enable Localized Translation in Reconstituted System",
  "title_text": "RNA Promotes Synapsin Condensates That Organize Synaptic Vesicles in Live Neurons and Enable Localized Translation in Reconstituted System",
  "type": "peer-reviewed",
  "updated_at": "2026-11-01T12:20:51Z",
  "url": "https://research.draconitic.com/paper/6xjn32wc4qhqh",
  "venue": "Advanced Science",
  "versions": []
}