{
  "abstract": "Heterotrimeric G proteins are key signal transducers in all eukaryotic cells. Anchored to the inner leaflet of the plasma membrane, they transmit and amplify extracellular chemical and physical signals downstream of G protein-coupled receptors. Despite numerous available studies, many biophysical aspects that regulate G protein signaling, including membrane mobility, remain poorly understood. Here, using single-molecule imaging, we show that different subtypes of heterotrimeric G proteins containing Gβ1γ2 subunits exhibit high diversity in their membrane mobility. We demonstrate that the nature of the Gα subunit plays a major role in defining the mobility of such heterotrimers. Our results indicate that heterotrimers containing Gα12 and Gα13 subunits exhibit markedly reduced mobility compared to those containing Gαi/o and Gαs subunits. These findings identify subtype-specific lateral membrane mobility of G proteins as a factor that can affect their signaling dynamics in living cells.",
  "abstract_shown": true,
  "affiliations": [
    "Faculty of Science, University of South Bohemia in České Budějovice, České Budějovice, Czechia",
    "Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, České Budějovice, Czechia",
    "Institute of Plant Molecular Biology, Biology Centre of the Czech Academy of Sciences, České Budějovice, Czechia"
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Ondřej Kuchynka"
    },
    {
      "affs": [
        1
      ],
      "name": "Andrei Kovalchuk"
    },
    {
      "affs": [
        2
      ],
      "name": "Mia Nußbaumer"
    },
    {
      "name": "Ekaterina Sviridova"
    },
    {
      "name": "Tomáš Fessl"
    },
    {
      "name": "Alexey Bondar"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6xq301angtpx1.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pone.0359304",
  "fulltext_url": null,
  "id": "6xq301angtpx1",
  "issns": [
    "1932-6203"
  ],
  "landing_url": "https://doi.org/10.1371/journal.pone.0359304",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Receptor Mechanisms and Signaling"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Lipids"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Proteins/Protein interactions"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Proteins/Transmembrane receptors/G protein coupled receptors"
    ],
    [
      "plos",
      "/Biology and life sciences/Cell biology/Cellular structures and organelles/Cell membranes"
    ],
    [
      "plos",
      "/Biology and life sciences/Cell biology/Cellular structures and organelles/Cell membranes/Membrane proteins"
    ],
    [
      "plos",
      "/Biology and life sciences/Cell biology/Cellular structures and organelles/Cell membranes/Membrane proteins/Integral membrane proteins"
    ],
    [
      "plos",
      "/Biology and life sciences/Cell biology/Signal transduction/Cell signaling/G-protein signaling"
    ],
    [
      "plos",
      "/Biology and life sciences/Cell biology/Signal transduction/Transmembrane receptors/G protein coupled receptors"
    ],
    [
      "plos",
      "/Physical sciences/Chemistry/Chemical physics/Mass diffusivity"
    ],
    [
      "plos",
      "/Physical sciences/Physics/Chemical physics/Mass diffusivity"
    ]
  ],
  "openalex": "W7214732184",
  "pdf_url": "https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0359304&type=printable",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-29",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/6xq301angtpx1.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:37Z",
      "native_id": "10.1371/journal.pone.0359304",
      "source": "plos",
      "url": "https://doi.org/10.1371/journal.pone.0359304"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "plos:cell biology",
      "subfield": "cell-biology"
    }
  ],
  "title": "Heterotrimeric G proteins containing Gβ₁γ₂ subunits exhibit subtype-specific mobility differences in live cells",
  "title_text": "Heterotrimeric G proteins containing Gβ₁γ₂ subunits exhibit subtype-specific mobility differences in live cells",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:37Z",
  "url": "https://research.draconitic.com/paper/6xq301angtpx1",
  "venue": "PLOS One",
  "versions": []
}