{
  "abstract": "Nucleolin (NCL) is a highly phosphorylated, multilocalized, pleiotropic nucleolar protein involved in chromatin organization, DNA damage response, the cell cycle, RNA metabolism, and ribosome biogenesis. A large-scale integration of publicly available human cellular phosphoproteome datasets led to the identification of 18 frequently detected/reported phosphosites across 1266 profiling and 507 differential datasets. Amidst various strategies currently being considered to target NCL in cancers, multi-domain distribution of these phosphosites differentially regulated across distinct cancers highlighted its plausible involvement in tumor-associated regulatory mechanisms. This demands the exploration of the potential kinases and interactors associated with these phosphorylation events. We performed a co-occurrence analysis to explore potential coordinated phosphorylation events among them and across other phosphoproteins. An integrative analysis of phosphosite-specific co-regulation, upstream kinases, and interaction networks was employed to explore potential phosphosite-specific signaling associations. Intriguingly, our analysis inferred the association of the predominant phosphosites within the acidic tract with chromatin organization and histone-related functions, whereas TPxKK motif phosphosites were linked to NCL localization, chromosome segregation, and nucleolar reorganization. Phosphosites in the RRM domain showed a potential association with RNA-binding proteins and metabolic pathways. Collectively, these findings suggest a domain-specific phosphosite-centric regulatory network of NCL, providing a valuable reference framework for future experimental studies and phosphosite-directed therapeutic strategies in cancers.",
  "abstract_shown": true,
  "affiliations": [
    "Centre for Integrative Omics Data Science (CIODS), Yenepoya (Deemed to Be University), Mangalore 575018, Karnataka, India"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Megha Shaji"
    },
    {
      "affs": [
        0
      ],
      "name": "Jaytha Thomas"
    },
    {
      "affs": [
        0
      ],
      "name": "Pathiyil Sajini Sekhar"
    },
    {
      "affs": [
        0
      ],
      "name": "Suhail Subair"
    },
    {
      "affs": [
        0
      ],
      "name": "Althaf Mahin"
    },
    {
      "affs": [
        0
      ],
      "name": "Leona Dcunha",
      "orcid": "0009-0004-2245-9522"
    },
    {
      "affs": [
        0
      ],
      "name": "Alimath Sambreena"
    },
    {
      "affs": [
        0
      ],
      "name": "Rajesh Raju",
      "orcid": "0000-0003-2319-121X"
    },
    {
      "affs": [
        0
      ],
      "name": "Sowmya Soman"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6xth975bvzr0f.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27209132",
  "fulltext_url": null,
  "id": "6xth975bvzr0f",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/20/9132",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1607|Advanced Proteomics Techniques and Applications"
    ]
  ],
  "openalex": "W7222065641",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-15",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/6xth975bvzr0f.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:55Z",
      "native_id": "10.3390/ijms27209132",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/20/9132"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:1607|Advanced Proteomics Techniques and Applications",
      "subfield": "physical"
    }
  ],
  "title": "Co-Regulation Analysis of Domain-Specific Phosphosites Reveals Insights into the Phosphoregulation-Directed Functional Diversity of Nucleolin",
  "title_text": "Co-Regulation Analysis of Domain-Specific Phosphosites Reveals Insights into the Phosphoregulation-Directed Functional Diversity of Nucleolin",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:55Z",
  "url": "https://research.draconitic.com/paper/6xth975bvzr0f",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}