{
  "abstract": "Antibiotics are widely used to treat bacterial infections, yet overuse and misuse causes disruption of the gut microbiome and spread of antibiotic-resistant bacteria. Presence of antibiotic resistance genes (ARGs) could be a determinant of the response to antibiotics. Supplementation with prebiotics may limit the detrimental antibiotic-induced effects on the microbiome, but whether this results in changes in the gut resistome is unclear. This study investigated the impact of eight-week 2'-fucosyllactose (2'-FL) supplementation after seven-day vancomycin use in 18 adults with overweight/obesity, a subset from a double-blind placebo-controlled randomized intervention. Whole-metagenome shotgun sequencing was used to characterize the gut microbiome and resistome at baseline, after vancomycin use and after supplementation. Seven-day vancomycin use disrupted microbial richness and diversity (p < 0.001) and changed microbial composition. No improvements in microbial recovery were detected after eight week 2'-FL supplementation compared to placebo. Resistome richness and diversity were not affected by vancomycin use, although the presence of specific ARGs changed. No differential impact of 2'-FL supplementation on resistome richness, diversity and composition was detected. While vancomycin use disrupted the gut microbiome and affected the resistome, the detectable effects of 2'-FL supplementation were limited. Future research should investigate antibiotic- and prebiotic-induced changes in the gut resistome in more detail.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Human Biology, NUTRIM Institute of Nutrition and Translational Research in Metabolism, Maastricht University Medical Center+, Maastricht, The Netherlands.",
    "Department of Medical Microbiology, Infectious Diseases and Infection Prevention, NUTRIM Institute of Nutrition and Translational Research in Metabolism, Maastricht University Medical Center+, Maastricht, The Netherlands."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Lars M M Vliex",
      "orcid": "0000-0002-5295-4223"
    },
    {
      "affs": [
        1
      ],
      "name": "Iúri F Godinho Mimoso",
      "orcid": "0009-0003-9994-6081"
    },
    {
      "affs": [
        1
      ],
      "name": "Giang N Le",
      "orcid": "0000-0001-6660-557X"
    },
    {
      "affs": [
        0
      ],
      "name": "Ellen E Blaak",
      "orcid": "0000-0002-2496-3464"
    },
    {
      "affs": [
        1
      ],
      "name": "John Penders",
      "orcid": "0000-0001-9146-5919"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6ybst4rhnxr9a.bib",
  "cited_by": 0,
  "doi": "10.1080/19490976.2026.2746814",
  "fulltext_url": "https://europepmc.org/articles/PMC13679327",
  "id": "6ybst4rhnxr9a",
  "issns": [
    "1949-0976",
    "1949-0984"
  ],
  "landing_url": "https://europepmc.org/article/MED/42878177",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Gut microbiota and health"
    ]
  ],
  "openalex": "W7222306834",
  "pdf_url": "https://europepmc.org/articles/PMC13679327?pdf=render",
  "pmcid": "PMC13679327",
  "pmid": "42878177",
  "published": "2026-10-15",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/6ybst4rhnxr9a.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:46Z",
      "native_id": "MED:42878177",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42878177"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Gut microbiota and health",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Modulation of the gut microbiome and resistome by antibiotic use, but not by subsequent 2'-fucosyllactose intake, in adults with overweight or obesity.",
  "title_text": "Modulation of the gut microbiome and resistome by antibiotic use, but not by subsequent 2'-fucosyllactose intake, in adults with overweight or obesity.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:46Z",
  "url": "https://research.draconitic.com/paper/6ybst4rhnxr9a",
  "venue": "Gut microbes",
  "versions": []
}