{
  "abstract": "目的: 子宫内膜异位症(endometriosis，EMs)病灶中富集的M2型巨噬细胞参与免疫逃逸及血管生成，异位内膜间质细胞的迁移能力亦与病灶进展密切相关，但二者是否受同一上游分子调控尚不清楚。本研究旨在探讨子宫内膜间质细胞来源的miR-423-5p是否通过靶向上皮膜蛋白2(epithelial membrane protein 2，EMP2)促进巨噬细胞的M2型极化，并直接增强间质细胞自身迁移能力，从而协同参与EMs病灶进展。\n\n方法: 从基因表达综合数据库(Gene Expression Omnibus，GEO)下载EMs相关数据集GSE7305，其中包括9例EMs异位子宫内膜及7例无EMs女性的正常子宫内膜组织，评估并比较22种免疫细胞的免疫浸润情况。收集EMs患者异位与在位内膜组织各10例，通过免疫组织化学染色、反转录PCR(reverse transcription PCR，RT-PCR)、蛋白质印迹法检测miR-423-5p、EMP2及M2型标志物的表达。采用双萤光素酶报告基因实验验证miR-423-5p与EMP2 3'-非翻译区(untranslated region，UTR)的直接靶向结合。体外诱导THP-1细胞分化为M2型巨噬细胞，分别转染miR-423-5p mimics、mimics阴性对照(mimics NC)、miR-423-5p inhibitor、inhibitor阴性对照(inhibitor NC)；同时，分离并培养原代人子宫内膜间质细胞(primary human endometrial stromal cells，phESCs)，转染后与M2型巨噬细胞共培养；采用RT-PCR、蛋白质印迹法检测miR-423-5p、EMP2、CD206及白细胞介素(interleukin，IL)-10的表达；采用划痕愈合实验检测phESCs的迁移能力。\n\n结果: 生物信息学分析结果显示，异位子宫内膜组织中M2型巨噬细胞的比例显著高于正常子宫内膜组织( P<0.05)。免疫组织化学染色及RT-PCR、蛋白质印迹法结果均显示，EMs患者异位子宫内膜组织中M2型标志物CD206和IL-10的表达水平均显著高于患者的在位子宫内膜，同时EMP2的表达水平显著低于在位子宫内膜(均 P<0.05)。RT-PCR检测结果显示，异位内膜来源的phESCs中miR-423-5p的表达水平显著高于在位内膜来源的phESCs( P<0.05)。双萤光素酶实验结果显示，miR-423-5p可直接结合EMP2的3'-UTR并抑制其活性( P<0.05)。在M2型巨噬细胞中过表达miR-423-5p能显著上调CD206和IL-10的表达水平，并下调EMP2的表达水平(均 P<0.05)；抑制miR-423-5p则产生相反作用。共培养实验进一步证实，过表达miR-423-5p的间质细胞可促进共培养体系中巨噬细胞的M2型极化并下调EMP2的表达(均 P<0.05)。划痕愈合实验显示，上调miR-423-5p表达可显著增强phESCs的迁移能力( P<0.05)。\n\n结论: 子宫内膜间质细胞可能通过分泌miR-423-5p，靶向下调巨噬细胞中EMP2的表达，从而驱动其向M2型极化，同时增强自身的迁移能力，两者共同参与EMs免疫微环境的重塑与病情的进展。",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "name": "美佳 蒋",
      "orcid": "0009-0006-8579-5771"
    },
    {
      "name": "静妮 吴",
      "orcid": "0000-0003-2781-8622"
    },
    {
      "name": "小玲 方"
    },
    {
      "name": "一帆 李"
    },
    {
      "name": "婷婷 章",
      "orcid": "0000-0001-6808-208X"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/6z2te8avdkpx8.bib",
  "cited_by": 0,
  "doi": "10.11817/j.issn.1672-7347.2026.260201",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13710914/",
  "id": "6z2te8avdkpx8",
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  "landing_url": "https://doi.org/10.11817/j.issn.1672-7347.2026.260201",
  "language": "zh",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
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      "2743|Endometriosis Research and Treatment"
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  "openalex": "W7223787045",
  "pdf_url": null,
  "pmcid": "PMC13710914",
  "pmid": "42935196",
  "published": "2026-08-28",
  "publisher": "Central South University",
  "ris": "https://research.draconitic.com/paper/6z2te8avdkpx8.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T12:21:47Z",
      "native_id": "PMC13710914",
      "source": "pmc",
      "url": "https://doi.org/10.11817/j.issn.1672-7347.2026.260201"
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  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2743|Endometriosis Research and Treatment",
      "subfield": "reproductive-child"
    }
  ],
  "title": "间质细胞来源的miR -423 -5p靶向EMP2促进子宫内膜异位症中M2型巨噬细胞极化并直接增强间质细胞迁移",
  "title_text": "间质细胞来源的miR -423 -5p靶向EMP2促进子宫内膜异位症中M2型巨噬细胞极化并直接增强间质细胞迁移",
  "type": "peer-reviewed",
  "updated_at": "2026-10-31T12:21:47Z",
  "url": "https://research.draconitic.com/paper/6z2te8avdkpx8",
  "venue": "Journal of Central South University Medical Sciences",
  "versions": []
}