{
  "abstract": "Understanding protein function requires tools to visualize and manipulate proteins in vivo . NanoTags, short epitope tags that are recognized by single-chain antibodies, are an emerging method for versatile in vivo labelling and manipulation of endogenous proteins. The short (13 amino acid) ALFA tag has been shown to allow labelling of endogenous proteins in several model systems, including C. elegans . Here we describe the generation and validation of transgenes to facilitate global and tissue-specific visualization of ALFA-tagged endogenous proteins in C. elegans . We propose a modular NanoTag system as a powerful approach for investigating protein functions in vivo in C. elegans .",
  "abstract_shown": true,
  "affiliations": [
    "Basic Sciences Division, Fred Hutchinson Cancer Center, Seattle, Washington, United States."
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 2,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Irini Topalidou"
    },
    {
      "affs": [
        0
      ],
      "name": "Nicolas Lehrbach"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7085qm2p4g1r9.bib",
  "cited_by": 0,
  "doi": "10.17912/micropub.biology.002419",
  "fulltext_url": "https://europepmc.org/articles/PMC13741013",
  "id": "7085qm2p4g1r9",
  "issns": [
    "2578-9430"
  ],
  "landing_url": "https://europepmc.org/article/MED/42997131",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1304|Advanced Fluorescence Microscopy Techniques"
    ]
  ],
  "openalex": "W7223084175",
  "pdf_url": "https://europepmc.org/articles/PMC13741013?pdf=render",
  "pmcid": "PMC13741013",
  "pmid": "42997131",
  "published": "2026-10-21",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/7085qm2p4g1r9.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T15:20:41Z",
      "native_id": "MED:42997131",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42997131"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1304|Advanced Fluorescence Microscopy Techniques",
      "subfield": "biophysics"
    }
  ],
  "title": "Tools for in vivo labelling of ALFA-tagged proteins in C. elegans .",
  "title_text": "Tools for in vivo labelling of ALFA-tagged proteins in C. elegans .",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T15:20:41Z",
  "url": "https://research.draconitic.com/paper/7085qm2p4g1r9",
  "venue": "MicroPublication biology",
  "versions": []
}