{ "abstract": "Background: White feces syndrome (WFS) is a major health problem affecting intensive shrimp aquaculture, but its underlying mechanisms remain unclear. Increasing evidence suggests that WFS may involve complex interactions among host physiological status, intestinal microbial dysbiosis, and environmental microbial communities. However, the relationships among these factors within commercial shrimp culture systems remain poorly understood. Objectives: This study aimed to investigate the associations among intestinal antioxidant capacity, intestinal microbiota, sediment microbial communities, and water quality parameters in Litopenaeus vannamei affected by WFS under small-pond culture conditions. Methods: Healthy and WFS-affected shrimp were collected from a commercial shrimp farm in Zhanjiang, Guangdong Province, China. Intestinal antioxidant parameters, including total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA), were determined. Intestinal and sediment microbial communities were analyzed using 16S rRNA gene sequencing. Water quality parameters and microbial community composition were compared between healthy and WFS groups. Results: Compared with healthy shrimp, WFS-affected shrimp exhibited significantly increased intestinal MDA levels and decreased activities of T-AOC, SOD, and GSH-Px, indicating an imbalance in antioxidant status. Significant differences in intestinal microbial community composition were observed between groups, as confirmed by beta-diversity analysis (PERMANOVA, R2 = 0.407, P = 0.022). WFS-associated microbial alterations included changes in dominant bacterial taxa and predicted microbial functional potential related to metabolism. The dominant taxa, referring to the most abundant microbial groups based on relative abundance, differed between healthy and WFS-affected shrimp. Sediment microbial communities also showed substantial compositional shifts, suggesting alterations in the surrounding microbial environment. In addition, nitrate and total nitrogen concentrations were significantly reduced in the WFS group. Conclusion: These findings indicate that WFS occurrence is associated with coordinated alterations in shrimp antioxidant status, intestinal microbial structure, sediment microbial communities, and environmental conditions. This study provides new insights into WFS from a host–microbiota–environment interaction perspective and highlights potential indicators for monitoring shrimp health in aquaculture systems.", "abstract_shown": true, "affiliations": [ "Guangdong Evergreen Feed industry Co., Ltd, Aquatic Feed Research Institute, Zhanjiang, Guangdong, China.", "Guangdong Provincial Key Laboratory of Evergreen Aquaculture, Research and Development Department, Zhanjiang, Guangdong, China.", "Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong, China.", "Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Guangdong Ocean University, College of Fisheries, Zhanjiang, Guangdong, China.", "Aquatic Animals Precision Nutrition and High-Efficiency Feed Engineering Research Center of Guangdong Province, Guangdong Ocean University, College of Fisheries, Zhanjiang, Guangdong, China.", "Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Guangdong Ocean University, College of Fisheries, Zhanjiang, Guangdong, China." ], "article_type": "Research Article", "arxiv": null, "author_total": 8, "authors": [ { "affs": [ 0, 1 ], "name": "Zhuoduo Wang" }, { "affs": [ 2, 3, 4 ], "name": "Xin Zhang" }, { "affs": [ 2, 3, 4 ], "name": "Kangze Xv" }, { "affs": [ 2, 3, 4 ], "name": "Adinda Luthfiah" }, { "affs": [ 0, 1 ], "name": "Yongjie Jiang" }, { "affs": [ 0, 1 ], "name": "Limei Zhao" }, { "affs": [ 2, 3, 4, 5 ], "name": "Beiping Tan" }, { "affs": [ 2, 3, 4, 5 ], "name": "Wei Zhang", "orcid": "0009-0005-3365-2739" } ], "bibtex": "https://research.draconitic.com/paper/70hmf700vfet5.bib", "cited_by": 0, "doi": "10.1371/journal.pone.0357891", "fulltext_url": "https://europepmc.org/articles/PMC13690251", "id": "70hmf700vfet5", "issns": [ "1932-6203" ], "landing_url": "https://doi.org/10.1371/journal.pone.0357891", "language": "eng", "licence": "CC-BY", "licence_label": "CC BY", "licence_source": "article", "licence_url": "https://creativecommons.org/licenses/by/4.0/", "metadata_licence": null, "native_subjects": [ [ "openalex", "2403|Aquaculture disease management and microbiota" ], [ "plos", "/Biology and life sciences/Anatomy/Digestive system/Gastrointestinal tract" ], [ "plos", "/Biology and life sciences/Biochemistry/Antioxidants" ], [ "plos", "/Biology and life sciences/Ecology/Ecological metrics/Species diversity" ], [ "plos", "/Biology and life sciences/Organisms/Bacteria/Gut bacteria" ], [ "plos", "/Biology and life sciences/Organisms/Eukaryota/Animals/Invertebrates/Arthropoda/Crustaceans/Shrimp" ], [ "plos", "/Biology and life sciences/Zoology/Animals/Invertebrates/Arthropoda/Crustaceans/Shrimp" ], [ "plos", "/Earth sciences/Geology/Petrology/Sediment" ], [ "plos", "/Earth sciences/Geology/Sedimentary geology/Sediment" ], [ "plos", "/Earth sciences/Marine and aquatic sciences/Bodies of water/Ponds" ], [ "plos", "/Ecology and environmental sciences/Ecology/Ecological metrics/Species diversity" ], [ "plos", "/Ecology and environmental sciences/Water quality" ], [ "plos", "/Medicine and health sciences/Anatomy/Digestive system/Gastrointestinal tract" ] ], "openalex": "W7223210047", "pdf_url": "https://europepmc.org/articles/PMC13690251?pdf=render", "pmcid": "PMC13690251", "pmid": "42911970", "published": "2026-10-22", "publisher": "Public Library of Science", "ris": "https://research.draconitic.com/paper/70hmf700vfet5.ris", "sources": [ { "first_seen": "2026-10-30T12:17:23Z", "native_id": "10.1371/journal.pone.0357891", "source": "plos", "url": "https://doi.org/10.1371/journal.pone.0357891" }, { "first_seen": "2026-10-30T12:20:10Z", "native_id": "MED:42911970", "source": "europepmc", "url": "https://europepmc.org/article/MED/42911970" } ], "subjects": [ { "field": "biology", "origin": "plos:ecology", "subfield": "ecology" }, { "field": "earth", "origin": "plos:geology", "subfield": "geology-geochemistry" }, { "field": "medicine", "origin": "plos:anatomy", "subfield": "physiology" } ], "title": "White Feces Syndrome is closely linked to impaired antioxidant capacity, the aquaculture environment, and microbial dysbiosis in Litopenaeus vannamei reared in small-pond culture system", "title_text": "White Feces Syndrome is closely linked to impaired antioxidant capacity, the aquaculture environment, and microbial dysbiosis in Litopenaeus vannamei reared in small-pond culture system", "type": "peer-reviewed", "updated_at": "2026-10-30T12:20:10Z", "url": "https://research.draconitic.com/paper/70hmf700vfet5", "venue": "PLOS One", "versions": [] }