{
  "abstract": "Dictyostelium discoideum is a key Amoebozoa model organism in cell biology, offering unique insights into chemotaxis, cell polarity and the transition from unicellular to multicellular life. Here, we evaluated the highly photostable fluorescent protein mStayGold for live-cell imaging in D. discoideum . mStayGold showed localization patterns comparable to Achilles when fused to Lifeact or the PKBR1(N150) membrane marker. Compared with Achilles, mStayGold exhibited greater resistance to photobleaching under continuous illumination and lower cell-to-cell variability in fluorescence intensity. These properties make mStayGold a promising probe for demanding imaging applications, including super-resolution microscopy, high-speed imaging, long-term developmental imaging and single-molecule tracking.",
  "abstract_shown": true,
  "affiliations": [
    "Graduate School of Arts and Sciences, The University of Tokyo, Meguro-ku, Tokyo, Japan.",
    "Research Center for Complex Systems Biology, Universal Biology Institute, The University of Tokyo, Meguro-ku, Tokyo, Japan."
  ],
  "article_type": "brief report",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Hidenori Hashimura"
    },
    {
      "affs": [
        0
      ],
      "name": "Shoko Fujishiro"
    },
    {
      "affs": [
        0
      ],
      "name": "Toyoko Sugita"
    },
    {
      "affs": [
        0
      ],
      "name": "Hibiki Nakagawa"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Satoshi Sawai"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/713tq02ge6rgj.bib",
  "cited_by": 0,
  "doi": "10.17912/micropub.biology.002300",
  "fulltext_url": "https://europepmc.org/articles/PMC13714645",
  "id": "713tq02ge6rgj",
  "issns": [
    "2578-9430"
  ],
  "landing_url": "https://europepmc.org/article/MED/42953504",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "D. Discoideum"
    ],
    [
      "heading",
      "Materials and Reagents"
    ],
    [
      "heading",
      "Methodology"
    ],
    [
      "heading",
      "Methods"
    ],
    [
      "openalex",
      "1304|Advanced Fluorescence Microscopy Techniques"
    ]
  ],
  "openalex": "W7221651618",
  "pdf_url": "https://europepmc.org/articles/PMC13714645?pdf=render",
  "pmcid": "PMC13714645",
  "pmid": "42953504",
  "published": "2026-10-13",
  "publisher": "California Institute of Technology",
  "ris": "https://research.draconitic.com/paper/713tq02ge6rgj.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T00:20:41Z",
      "native_id": "MED:42953504",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42953504"
    },
    {
      "first_seen": "2026-11-01T12:22:06Z",
      "native_id": "PMC13714645",
      "source": "pmc",
      "url": "https://doi.org/10.17912/micropub.biology.002300"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1304|Advanced Fluorescence Microscopy Techniques",
      "subfield": "biophysics"
    }
  ],
  "title": "Use of photostable green fluorescent protein mStayGold for live-cell imaging in Dictyostelium discoideum.",
  "title_text": "Use of photostable green fluorescent protein mStayGold for live-cell imaging in Dictyostelium discoideum.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-01T12:22:06Z",
  "url": "https://research.draconitic.com/paper/713tq02ge6rgj",
  "venue": "MicroPublication biology",
  "versions": []
}