{
  "abstract": "Adsorbing antibodies of interest onto solid supports displaying corresponding antigens is an efficient strategy for their subsequent purification and detection. We have previously demonstrated the potential of water-dispersible, antibiofouling, and designable cello-oligosaccharide assemblies for antibody detection via enzyme-linked immunosorbent assay (ELISA). However, whether antigen-conjugated cello-oligosaccharide assemblies can detect specific antibodies contained in immune serum remains unexplored. In this study, we demonstrate the utility of oligo(ethylene glycol) (OEG)-conjugated cello-oligosaccharide assemblies for detecting antipoly(ethylene glycol) (PEG) immunoglobulin M (IgM) in serum from immunized animals. Mouse sera containing anti-PEG IgMs, induced by immunogenic polymers, were utilized for evaluation. ELISA results revealed that anti-PEG IgMs in the serum with no heat-inactivation or depletion were successfully detected by cello-oligosaccharide assemblies displaying OEG chains with at least three repeating ethylene glycol units. ELISAs using a series of cello-oligosaccharide assemblies displaying OEG chains indicated that the conjugation rate of OEG chains influences anti-PEG IgM binding. Furthermore, increasing the serum concentration led to enhanced detection efficacy for anti-PEG IgM, likely due to the superior antibiofouling properties of the cello-oligosaccharide assemblies, which minimize nonspecific adsorption of serum proteins. The concentration of the assemblies also significantly influenced the detection efficacy. Additionally, 7 days after a single intravenous injection of OEG-conjugated cello-oligosaccharide assemblies without micrometer-scale aggregates, ELISA signals for IgMs against PEG chains, OEG chains, and cello-oligosaccharide moieties were comparable to those of saline-treated controls. These findings demonstrated that OEG-conjugated cello-oligosaccharide assemblies hold considerable potential for the detection of anti-PEG IgMs in immune serum with no heat-inactivation or depletion.",
  "abstract_shown": true,
  "affiliations": [
    "Institute of Science Tokyo , , 2-12-1-H-121 Ookayama , Meguro-ku, ,",
    "Juntendo University , , 6-8-1 Hinode , , ,",
    "the Jikei University School of Medicine , , 163-1 Kashiwa-shita , , ,"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Kai Sugiura",
      "orcid": "0009-0009-0912-2171"
    },
    {
      "affs": [
        0
      ],
      "name": "Yuuki Hata",
      "orcid": "0000-0003-1493-1896"
    },
    {
      "affs": [
        1
      ],
      "name": "Hiroshi Tanaka"
    },
    {
      "affs": [
        2
      ],
      "name": "Kouichi Shiraishi",
      "orcid": "0009-0003-5097-4567"
    },
    {
      "affs": [
        0
      ],
      "name": "Takeshi Serizawa",
      "orcid": "0000-0002-4867-8625"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/72p4z6szs7azs.bib",
  "cited_by": 0,
  "doi": "10.1021/acsomega.6c04925",
  "fulltext_url": null,
  "id": "72p4z6szs7azs",
  "issns": [
    "2470-1343"
  ],
  "landing_url": "https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c04925/5466299/Detection-of-Antipoly-ethylene-glycol-IgM-in",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": "https://pubs.acs.org/doi/pdf/10.1021/acsomega.6c04925",
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-09",
  "publisher": "American Chemical Society (ACS)",
  "ris": "https://research.draconitic.com/paper/72p4z6szs7azs.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T12:22:10Z",
      "native_id": "10.1021/acsomega.6c04925",
      "source": "crossref",
      "url": "https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c04925/5466299/Detection-of-Antipoly-ethylene-glycol-IgM-in"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "issn:2470-1343",
      "subfield": "general"
    }
  ],
  "title": "Detection of Antipoly(ethylene glycol) IgM in Immune Serum by Oligo(ethylene glycol)-Conjugated Cello-oligosaccharide Assemblies",
  "title_text": "Detection of Antipoly(ethylene glycol) IgM in Immune Serum by Oligo(ethylene glycol)-Conjugated Cello-oligosaccharide Assemblies",
  "type": "peer-reviewed",
  "updated_at": "2026-11-10T00:21:21Z",
  "url": "https://research.draconitic.com/paper/72p4z6szs7azs",
  "venue": "ACS Omega",
  "versions": []
}