{
  "abstract": "Brucella abortus is an intracellular pathogen that profoundly alters macrophage responses, but the contribution and extracellular distribution of long non-coding RNAs (lncRNAs) during infection remain poorly characterized. Here, we characterized the exosomal lncRNA landscape of B. abortus-infected RAW 264.7 macrophages and applied an integrated bioinformatic framework to prioritize candidate lncRNAs and their potential functional contexts. Exosomes were isolated from infected and control macrophages at 8 h post-infection, and their RNA content was analyzed by high-throughput sequencing. Differential expression analysis identified 83 lncRNAs with altered expression, with most showing reduced expression in exosomes from infected cells. Functional enrichment analysis of protein-coding genes located within ±250 kb of the differentially expressed lncRNAs generated associations with immune-related processes, including antigen presentation and cytotoxicity, as well as ion transport, lipid metabolism, and lymphocyte-related processes. A complementary Enrichr analysis provided complementary immune-related signals, although these did not remain significant after multiple-testing corrections. Malat1, Neat1, and Gas5 were selected for RT-qPCR validation and showed expression patterns consistent with sequencing. AlphaFold 3 predicted Snu13 as a potential interactor of the three candidate lncRNAs, providing a hypothesis for future experimental investigation but not establishing a role in exosomal RNA sorting. Overall, these findings provide a transcriptomic resource and computational framework for prioritizing exosomal lncRNAs associated with B. abortus infection and support further investigation of EV-associated lncRNA loading mechanism and their potential functional relevance.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "Original Research",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "name": "Francisco Álvarez"
    },
    {
      "name": "Emilio Gutiérrez"
    },
    {
      "name": "Ítalo Ferrari"
    },
    {
      "name": "Vicente Arriagada"
    },
    {
      "name": "Sebastián Ruiz"
    },
    {
      "name": "Belén Díaz"
    },
    {
      "name": "Leonardo A. Gómez"
    },
    {
      "name": "Ángel A. Oñate"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/72wy7sxf1jct6.bib",
  "cited_by": 0,
  "doi": "10.3389/fimmu.2026.1978629",
  "fulltext_url": null,
  "id": "72wy7sxf1jct6",
  "issns": [
    "1664-3224"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fimmu.2026.1978629",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "feed-journal",
      "biology/immunology"
    ]
  ],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-03",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/72wy7sxf1jct6.ris",
  "sources": [
    {
      "first_seen": "2026-11-03T06:20:16Z",
      "native_id": "10.3389/fimmu.2026.1978629",
      "source": "feed-frontiers",
      "url": "https://www.frontiersin.org/articles/10.3389/fimmu.2026.1978629"
    },
    {
      "first_seen": "2026-11-03T12:21:20Z",
      "native_id": "10.3389/fimmu.2026.1978629",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fimmu.2026.1978629/full"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "feed-journal:biology/immunology",
      "subfield": "immunology"
    }
  ],
  "title": "Early Brucella abortus infection reshapes the exosomal lncRNA cargo of macrophages and highlights putative immunoregulatory pathways",
  "title_text": "Early Brucella abortus infection reshapes the exosomal lncRNA cargo of macrophages and highlights putative immunoregulatory pathways",
  "type": "peer-reviewed",
  "updated_at": "2026-11-05T00:21:02Z",
  "url": "https://research.draconitic.com/paper/72wy7sxf1jct6",
  "venue": "Frontiers in Immunology",
  "versions": []
}