{
  "abstract": "Microgravity alters vascular homeostasis by disrupting mechanical cues critical to endothelial function. Here, we report a benchtop bioreactor that integrates simulated microgravity with physiological laminar shear stress ( ~ 3 dyn/cm²) to examine acute endothelial responses (16 h exposure). Using human umbilical vein endothelial cells (HUVECs), we observed cytoskeletal disorganization, loss of vascular endothelial cadherin junctions, and altered secretion of angiogenic factors, including Angiopoietin 2 (Angpt-2), Vascular Endothelial Growth Factor (VEGF), and Platelet-Derived Growth Factor (PDGF). This dual-stimulus platform bridges static microgravity and flow-driven endothelial models, enabling mechanistic studies of vascular dysfunction in spaceflight-relevant conditions and offering a framework for standardized microgravity-flow experimentation.",
  "abstract_shown": true,
  "affiliations": [
    "Ben Towne Center for Childhood Cancer Research, Seattle Children’s Hospital, Seattle, WA USA",
    "J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL USA",
    "Department of Materials Science & Engineering, University of Florida, Gainesville, FL USA",
    "Department of Pediatrics, Medical College of Wisconsin, Milwaukee, WI USA"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Nikolaos Pipis"
    },
    {
      "affs": [
        1
      ],
      "name": "Rachel Garner"
    },
    {
      "affs": [
        1
      ],
      "name": "Christopher Ludtka"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Josephine B. Allen"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/73g7kc4v0qcqa.bib",
  "cited_by": 1,
  "doi": "10.1038/s41526-026-00627-8",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13736950/",
  "id": "73g7kc4v0qcqa",
  "issns": [
    "2373-8065"
  ],
  "landing_url": "https://doi.org/10.1038/s41526-026-00627-8",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "2737|Spaceflight effects on biology"
    ]
  ],
  "openalex": "W7168028631",
  "pdf_url": null,
  "pmcid": "PMC13736950",
  "pmid": "42436195",
  "published": "2026-07-11",
  "publisher": "Nature Publishing Group",
  "ris": "https://research.draconitic.com/paper/73g7kc4v0qcqa.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T12:22:17Z",
      "native_id": "PMC13736950",
      "source": "pmc",
      "url": "https://doi.org/10.1038/s41526-026-00627-8"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2737|Spaceflight effects on biology",
      "subfield": "physiology"
    }
  ],
  "title": "Simulated microgravity with physiological shear reveals endothelial disruption in a dual-stimulus bioreactor",
  "title_text": "Simulated microgravity with physiological shear reveals endothelial disruption in a dual-stimulus bioreactor",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T12:22:17Z",
  "url": "https://research.draconitic.com/paper/73g7kc4v0qcqa",
  "venue": "NPJ Microgravity",
  "versions": []
}