{
  "abstract": "This study presents the first comprehensive in silico genome-wide identification, characterization, and mRNA expression profiling of the WRKY transcription factor (TF) gene family in Salvia hispanica L. (chia), a nutritious crop of the family Lamiaceae. A total of 35 CsWRKY genes were identified from the chia reference genome and classified into 7 phylogenetic groups (I, IIa–IIe, III) based on WRKY domain number and zinc-finger motif type. Mean protein length, molecular weight and isoelectric point were 332.8 aa, 37,499 Da and 7.62, respectively, and protein length was almost perfectly correlated with molecular weight (r = 0.9995). Subcellular-localization prediction indicated predominantly nuclear and cytoplasmic distribution. Gene-structure analysis revealed 2–7 exons, and MEME identified 15 conserved motifs including the invariant WRKYGQK heptapeptide. Segmental duplication predominated and has evolved under purifying selection. Promoter analysis detected 20 cis-acting regulatory element classes, and a maximum-likelihood phylogeny grouped CsWRKYs with orthologs from Arabidopsis thaliana, Oryza sativa, Glycine max, Medicago truncatula and Physcomitrella patens. In silico expression profiling used only verified, publicly available S. hispanica RNA-Seq datasets and was therefore restricted to tissue-specific and developmental patterns; because no abiotic-stress RNA-Seq dataset is currently available for chia, no stress-responsive expression analysis was performed. Homology models were built and docked with in silico predictions identifying CsWRKY3 as the strongest binder. These in silico predictions provide a preliminary genomic framework that requires future experimental validation and may guide the study of WRKY-mediated processes in chia.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Integrated and Advanced Technology, School of Engineering and Sciences, Sir Padampat Singhania University"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 1,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Shivani Tiwari"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/742yj1pmj6a1n.bib",
  "cited_by": 0,
  "doi": "10.1007/s44372-026-00972-8",
  "fulltext_url": "https://doi.org/10.1007/s44372-026-00972-8",
  "id": "742yj1pmj6a1n",
  "issns": [
    "3005-1207"
  ],
  "landing_url": "https://doi.org/10.1007/s44372-026-00972-8",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "QK1-989"
    ],
    [
      "lcc",
      "SB1-1110"
    ],
    [
      "openalex",
      "1312|Plant Gene Expression Analysis"
    ]
  ],
  "openalex": "W7223558846",
  "pdf_url": "https://link.springer.com/content/pdf/10.1007/s44372-026-00972-8.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-25",
  "publisher": "Springer Nature",
  "ris": "https://research.draconitic.com/paper/742yj1pmj6a1n.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:46:12Z",
      "native_id": "cbfe5b896ca64115a15cb97421f4dfe3",
      "source": "doaj",
      "url": "https://doi.org/10.1007/s44372-026-00972-8"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Plant Gene Expression Analysis",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Genome wide identification and in silico mRNA expression profiling of the WRKY transcription factor gene family in Salvia hispanica",
  "title_text": "Genome wide identification and in silico mRNA expression profiling of the WRKY transcription factor gene family in Salvia hispanica",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T13:46:12Z",
  "url": "https://research.draconitic.com/paper/742yj1pmj6a1n",
  "venue": "Discover Plants",
  "versions": []
}