{
  "abstract": "Different approaches to skeletal muscle regeneration using progenitor cells are extensively studied as strategies for the treatment of muscle tissue pathologies. Recently, it was discovered that anatomically localized alveolar mucosa multipotent mesenchymal stromal cells (AMCs) are characterized by myogenic potential and high feasibility for muscle tissue recovery and regeneration. Although the resulting multinuclear myotubes express skeletal muscle-specific markers (skeletal myosin, actin, myogenin, and MyoD1), the exact molecular mechanism controlling myogenic differentiation of AMCs is still unclear and poorly scrutinized. In this research, we used a combination of large-scale transcriptome analysis and a bioinformatics approach to investigate molecular pathways and crucial nodes responsible for myogenic differentiation. We studied differentiation of AMC cells in 2D and 3D culture conditions and found core genes with significant expression changes during differentiation and compared them with skeletal muscle-derived stromal cells (SMCs). It appeared that differentiation of AMCs in 3D is significantly different from that in 2D. Moreover, differentiation of AMCs in 2D is closer to differentiation of SMCs in 2D than to AMCs in 3D. Unique properties of AMC differentiation in 3D may be attributed to a significant inhibition of the component of the PDGFRβ signaling pathway responsible for ruffle organization.",
  "abstract_shown": true,
  "affiliations": [
    "Institute of Personalized Oncology, I.M. Sechenov First Moscow State Medical University, Moscow 119991, Russia.",
    "Omicsway Corp., 340 S Lemon Ave, 6040, Walnut, CA 91789, USA.",
    "Oncobox Ltd., Moscow 121205, Russia.",
    "Sirius University of Science and Technology, 1 Olimpiyskiy Ave, Sirius 354340, Krasnodar Region, Russia.",
    "Petrovsky National Research Center of Surgery, Moscow 119991, Russia.",
    "Pirogov National Medical and Surgical Center, Moscow 105203, Russia.",
    "Institute for Regenerative Medicine, I.M. Sechenov First Moscow State Medical University, Moscow 119991, Russia.",
    "Artgen Biotech, Moscow 119333, Russia.",
    "Skincell LLC, Moscow 119333, Russia.",
    "Department of Pharmacology, I.M. Sechenov First Moscow State Medical University, Moscow 119991, Russia.",
    "Department of Pharmacognosy and Industrial Pharmacy, Lomonosov Moscow State University, Moscow 119992, Russia.",
    "Department of Pathology, Kazan State Medical University, Kazan 420012, Russia.",
    "Department of Radiotherapy and Radiology, Russian Medical Academy of Continuous Professional Education, Moscow 119454, Russia.",
    "N.N. Blokhin National Medical Research Center of Oncology, Moscow 115522, Russia."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 17,
  "authors": [
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Maksim Sorokin",
      "orcid": "0000-0001-7685-3446"
    },
    {
      "affs": [
        0,
        1,
        2,
        3
      ],
      "name": "Anton Buzdin"
    },
    {
      "affs": [
        0
      ],
      "name": "Anastasia Guryanova"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Alexander Gudkov"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Alexander Modestov",
      "orcid": "0000-0002-1531-7161"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Maria Suntsova"
    },
    {
      "affs": [
        4
      ],
      "name": "Ilya Eremin"
    },
    {
      "affs": [
        5
      ],
      "name": "Andrey Pulin",
      "orcid": "0000-0002-9499-4979"
    },
    {
      "affs": [
        6
      ],
      "name": "Nastasia Kosheleva"
    },
    {
      "affs": [
        7,
        8
      ],
      "name": "Alla Zorina"
    },
    {
      "affs": [
        7,
        8
      ],
      "name": "Vadim Zorin"
    },
    {
      "affs": [
        9,
        10
      ],
      "name": "Dmitry Kudlay"
    },
    {
      "affs": [
        11,
        12
      ],
      "name": "Sergei Boichuk",
      "orcid": "0000-0003-2415-1084"
    },
    {
      "affs": [
        13
      ],
      "name": "Maria Vasileva"
    },
    {
      "affs": [
        13
      ],
      "name": "Maria Fedorova"
    },
    {
      "affs": [
        13
      ],
      "name": "Svetlana Vinokurova"
    },
    {
      "affs": [
        13
      ],
      "name": "Pavel Kopnin",
      "orcid": "0000-0002-2078-4274"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/75f7gpbbnhcxd.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27198712",
  "fulltext_url": "https://europepmc.org/articles/PMC13665378",
  "id": "75f7gpbbnhcxd",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://europepmc.org/article/MED/42883956",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Muscle Physiology and Disorders"
    ]
  ],
  "openalex": "W7214796014",
  "pdf_url": "https://europepmc.org/articles/PMC13665378?pdf=render",
  "pmcid": "PMC13665378",
  "pmid": "42883956",
  "published": "2026-09-29",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/75f7gpbbnhcxd.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:16Z",
      "native_id": "MED:42883956",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42883956"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Muscle Physiology and Disorders",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Molecular Mechanisms Implicated in Myogenic Differentiation of Human Alveolar Mucosa-Derived Cells.",
  "title_text": "Molecular Mechanisms Implicated in Myogenic Differentiation of Human Alveolar Mucosa-Derived Cells.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:16Z",
  "url": "https://research.draconitic.com/paper/75f7gpbbnhcxd",
  "venue": "International journal of molecular sciences",
  "versions": []
}