{
  "abstract": "α-l-Rhamnosidase is a key biocatalyst for glycosidic bond hydrolysis. Icariside I is a high-value flavonoid glycoside with enhanced bioavailability. However, its production from icariin is limited by enzyme stability and suboptimal catalytic performance. Here, an α-l-rhamnosidase mutant (R783A) was heterologously expressed, purified, and immobilized onto UiO-66-NH2via a simple adsorption strategy to construct a robust biocatalyst. The resulting R783A@UiO-66-NH2 exhibited markedly improved stability, including broad pH tolerance (6.0–9.0), retained more than 90% of its initial activity after 2 h at 60 °C, enhanced storage stability (88.6% residual activity after 30 days), and improved resistance to metal ions and organic solvents. Notably, R783A@UiO-66-NH2 efficiently catalyzed the hydrolysis of icariin, achieving a 99.8% conversion and a 97.6% icariside I yield within 5 h, corresponding to a space-time yield of 0.52 g L−1 h−1. In addition, the immobilized biocatalyst retained 85.6% yield after five consecutive reuse cycles and 60.3% after eight cycles at 60 °C, demonstrating useful operational stability and recyclability. This study provides a simple and efficient approach for the biotransformation of icariside I, with UiO-66-NH2 nanoparticles offering a versatile platform for high-value flavonoid production.",
  "abstract_shown": true,
  "affiliations": [
    "School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046 China"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Haoyu Jia",
      "orcid": "0009-0006-9725-2352"
    },
    {
      "affs": [
        0
      ],
      "name": "Tong Yan"
    },
    {
      "affs": [
        0
      ],
      "name": "Luran Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Lingling Song"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/77fa81026tga2.bib",
  "cited_by": 0,
  "doi": "10.1039/d6ra06339j",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13657089/?tool=EBI",
  "id": "77fa81026tga2",
  "issns": [
    "2046-2069"
  ],
  "landing_url": "https://europepmc.org/article/PMC/PMC13657089",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Chemistry"
    ],
    [
      "openalex",
      "1312|Enzyme Catalysis and Immobilization"
    ]
  ],
  "openalex": "W7221524950",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13657089/pdf/?tool=EBI",
  "pmcid": "PMC13657089",
  "pmid": "42866621",
  "published": "2026-10-12",
  "publisher": "Royal Society of Chemistry",
  "ris": "https://research.draconitic.com/paper/77fa81026tga2.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:29Z",
      "native_id": "PMC:PMC13657089",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13657089"
    },
    {
      "first_seen": "2026-11-01T12:23:36Z",
      "native_id": "PMC13657089",
      "source": "pmc",
      "url": "https://doi.org/10.1039/d6ra06339j"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Enzyme Catalysis and Immobilization",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Valorization of icariin via α-l-rhamnosidase mutant immobilized on UiO-66-NH2: an efficient and recyclable biocatalytic system for icariside I production",
  "title_text": "Valorization of icariin via α-l-rhamnosidase mutant immobilized on UiO-66-NH2: an efficient and recyclable biocatalytic system for icariside I production",
  "type": "peer-reviewed",
  "updated_at": "2026-11-02T12:20:50Z",
  "url": "https://research.draconitic.com/paper/77fa81026tga2",
  "venue": "RSC advances",
  "versions": []
}