{
  "abstract": "Protocol for monitoring cytoplasmic Ca 2+ dynamics using Fura Red-AM on a Zeiss Axio Observer microscope\n\nHere, we present a protocol for live-cell calcium imaging in HEK, HeLa, fibroblast, and myoblast cells using Fura Red-AM and a Zeiss Axio Observer inverted microscope. We describe steps for dye preparation and loading, microscope setup, dual-excitation image acquisition, and ratiometric signal analysis. The setup is optimized for store-operated calcium entry (SOCE), based on the activation of STIM1 and ORAI1. This protocol enables real-time monitoring of intracellular calcium dynamics and supports both non-transfected and GFP-transfected cells.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 2,
  "authors": [
    {
      "name": "Sabrina Beaumier"
    },
    {
      "name": "Marc Bartoli"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7akddcypqr2fb.bib",
  "cited_by": 0,
  "doi": "10.1016/j.xpro.2026.104469",
  "fulltext_url": null,
  "id": "7akddcypqr2fb",
  "issns": [
    "2666-1667"
  ],
  "landing_url": "https://hal.science/hal-05759938v1",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "hal",
      "1.sdv.gen"
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  "openalex": "W7147735147",
  "pdf_url": "https://hal.science/hal-05759938/document",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-23",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/7akddcypqr2fb.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:39Z",
      "native_id": "hal-05759938",
      "source": "hal",
      "url": "https://hal.science/hal-05759938v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "hal:1.sdv.gen",
      "subfield": "genetics-genomics"
    },
    {
      "field": "medicine",
      "origin": "hal:2.sdv.gen.gh",
      "subfield": "human-genetics"
    }
  ],
  "title": "Protocol for monitoring cytoplasmic Ca2+ dynamics using Fura Red-AM on a Zeiss Axio Observer microscope",
  "title_text": "Protocol for monitoring cytoplasmic Ca2+ dynamics using Fura Red-AM on a Zeiss Axio Observer microscope",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:39Z",
  "url": "https://research.draconitic.com/paper/7akddcypqr2fb",
  "venue": "STAR Protocols",
  "versions": []
}