{
  "abstract": "The voltage-gated potassium channel Ether-à-go-go 1 (EAG1) is aberrantly expressed in many cancers, yet its mechanistic contributions to tumor growth and metastasis in non–small cell lung cancer (NSCLC) remains poorly defined. Here, we establish a genetic EAG1 knockout in A549 NSCLC cells and demonstrate that loss of EAG1 markedly suppresses tumor growth and metastasis using a combination of tissue culture, xenograft, and multi-omics approaches. EAG1 knockout reduced proliferative ability across multiple in vitro assays and in vivo zebrafish xenografts. Furthermore, loss of EAG1 induced G1-phase cell-cycle arrest, accompanied by broad suppression of cell cycle regulators. Additionally, knocking out EAG1 significantly reduced migratory and invasive properties in vitro and markedly impaired extravasation in vivo. Integrated transcriptomic and proteomic analyses revealed that EAG1 regulates E2F signaling and epithelial–mesenchymal transition (EMT). Consistent with this, EAG1 knockout attenuated transforming growth factor-beta–induced EMT by disrupting the cadherin switch, a key mechanism for EMT, and abolished EMT-driven migration, demonstrating that EAG1 is required for the acquisition of a migratory phenotype. Together, these findings establish EAG1 as a central regulator of proliferation and metastasis of A549 cells, highlighting EAG1 as a potential therapeutic target in NSCLC.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Pharmacology and Physiology, Georgetown University Medical Center, Washington, District of Columbia, USA",
    "Department of Oncology, Georgetown University Medical Center, Washington, District of Columbia, USA"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Joos Berghausen"
    },
    {
      "affs": [
        1
      ],
      "name": "Emre Deniz"
    },
    {
      "affs": [
        0
      ],
      "name": "Ze-Jun Wang"
    },
    {
      "affs": [
        1
      ],
      "name": "Marcel Schmidt"
    },
    {
      "affs": [
        1
      ],
      "name": "Junfeng Ma"
    },
    {
      "affs": [
        1
      ],
      "name": "Karen Creswell"
    },
    {
      "affs": [
        1
      ],
      "name": "Aykut Üren"
    },
    {
      "affs": [
        1
      ],
      "name": "Eric Glasgow"
    },
    {
      "affs": [
        1
      ],
      "name": "Anton Wellstein"
    },
    {
      "affs": [
        0
      ],
      "name": "Tinatin I. Brelidze"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7ckwvarmv4q19.bib",
  "cited_by": 0,
  "doi": "10.1016/j.jbc.2026.113590",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13720809/",
  "id": "7ckwvarmv4q19",
  "issns": [
    "0021-9258",
    "1083-351X"
  ],
  "landing_url": "https://doi.org/10.1016/j.jbc.2026.113590",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research Article"
    ],
    [
      "openalex",
      "1312|Ion channel regulation and function"
    ]
  ],
  "openalex": "W7214064890",
  "pdf_url": null,
  "pmcid": "PMC13720809",
  "pmid": "42772558",
  "published": "2026-09-23",
  "publisher": "American Society for Biochemistry and Molecular Biology",
  "ris": "https://research.draconitic.com/paper/7ckwvarmv4q19.ris",
  "sources": [
    {
      "first_seen": "2026-11-03T12:21:37Z",
      "native_id": "PMC13720809",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.jbc.2026.113590"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Ion channel regulation and function",
      "subfield": "molecular-biology"
    }
  ],
  "title": "EAG1 channel is a central regulator of tumor growth and metastasis in A549 non-small cell lung cancer cells",
  "title_text": "EAG1 channel is a central regulator of tumor growth and metastasis in A549 non-small cell lung cancer cells",
  "type": "peer-reviewed",
  "updated_at": "2026-11-03T12:21:37Z",
  "url": "https://research.draconitic.com/paper/7ckwvarmv4q19",
  "venue": "The Journal of Biological Chemistry",
  "versions": []
}