{
  "abstract": "Laboratory cultivation imposes selective pressures that drive rapid bacterial evolution, compromising experimental reproducibility. Here, comparative phenotypic and genomic analyses revealed significant divergence between two Rhodospirillum rubrum S1 lineages originally obtained from the same culture collection. Although both shared baseline variations relative to the reference genome, their trajectories diverged. A recently sourced line (DSM 467) exhibited few additional mutations, including a fliF frameshift abolishing motility. Conversely, a long-maintained laboratory line remained motile but harbored numerous unique variants, particularly within stress-responsive histidine kinases. These results demonstrate passaging-induced genomic drift, highlighting the necessity of cryopreservation and minimal serial cultivation to ensure strain fidelity.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biology, Marburg University, Marburg, Germany.",
    "Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.",
    "Microbes-for-Climate (M4C) Cluster of Excellence & SYNMIKRO, Marburg, Germany."
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Sebastian Pöhl"
    },
    {
      "affs": [
        1
      ],
      "name": "Anke Treuner-Lange"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Martin Thanbichler"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7cr4znyh9rs5f.bib",
  "cited_by": 0,
  "doi": "10.17912/micropub.biology.002395",
  "fulltext_url": "https://europepmc.org/articles/PMC13741011",
  "id": "7cr4znyh9rs5f",
  "issns": [
    "2578-9430"
  ],
  "landing_url": "https://europepmc.org/article/MED/42997190",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1311|Bacterial Genetics and Biotechnology"
    ]
  ],
  "openalex": "W7223128984",
  "pdf_url": "https://europepmc.org/articles/PMC13741011?pdf=render",
  "pmcid": "PMC13741011",
  "pmid": "42997190",
  "published": "2026-10-20",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/7cr4znyh9rs5f.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T15:20:41Z",
      "native_id": "MED:42997190",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42997190"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1311|Bacterial Genetics and Biotechnology",
      "subfield": "genetics-genomics"
    }
  ],
  "title": "Comparative genomics of Rhodospirillum rubrum S1 lineages reveals significant differences from the reference genome.",
  "title_text": "Comparative genomics of Rhodospirillum rubrum S1 lineages reveals significant differences from the reference genome.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T15:20:41Z",
  "url": "https://research.draconitic.com/paper/7cr4znyh9rs5f",
  "venue": "MicroPublication biology",
  "versions": []
}