{
  "abstract": "Tissue morphogenesis integrates developmental programs with cell mechanics, yet the mechanisms coupling these processes remain poorly understood. We investigated this link during neuroepithelial morphogenesis in human iPSC-derived dorsal spinal organoids. We found that PAX3, a transcription factor whose loss causes neural tube defects, promotes force-bearing adherens junction assembly. It does so by driving apical clustering of N-cadherin complexes, thereby enabling neuroepithelial morphogenesis, a function conserved in the mouse neural tube. Transcriptomic, metabolomic, and functional analyses show that PAX3 sustains upper glycolysis and the associated mannose-dependent glycosylation pathways. Exogenous mannose rescues cadherin clustering and adherens junction mechanics following PAX3 loss, whereas inhibition of glycolysis or glycosylation recapitulates these defects. These findings establish mannose metabolism as a PAX3-regulated pathway conferring mechanical competence to developing tissues.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 22,
  "authors": [
    {
      "name": "Camil Mirdass"
    },
    {
      "name": "Lucas Denis"
    },
    {
      "name": "Hugo Lachuer"
    },
    {
      "name": "Mikaëlle Bocel"
    },
    {
      "name": "Anis Bourou"
    },
    {
      "name": "Kamal Bouhali"
    },
    {
      "name": "Nicolas Valentin"
    },
    {
      "name": "Sandrine Adiba"
    },
    {
      "name": "Alice Marteil"
    },
    {
      "name": "Céline Chollet"
    },
    {
      "name": "Emeline Chu-Van"
    },
    {
      "name": "Xavier Baudin"
    },
    {
      "name": "Catherine Pioche-Durieu"
    },
    {
      "name": "Sarah Chebouti"
    },
    {
      "name": "Bruno Estebe"
    },
    {
      "name": "Frédéric Relaix"
    },
    {
      "name": "Florence Castelli"
    },
    {
      "name": "Valérie Dupe"
    },
    {
      "name": "Sébastien Léon"
    },
    {
      "name": "Stéphane Nedelec"
    },
    {
      "name": "Nicolas Borghi"
    },
    {
      "name": "Vanessa Ribes"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7e5b65fjy7f3f.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.22.753382",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.22.753382v1.full",
  "id": "7e5b65fjy7f3f",
  "issns": [],
  "landing_url": "https://hal.science/hal-05775817v1",
  "language": "en",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "developmental biology"
    ],
    [
      "hal",
      "0.sdv"
    ],
    [
      "openalex",
      "1312|Developmental Biology and Gene Regulation"
    ]
  ],
  "openalex": "W7214238298",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.22.753382v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-22",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/7e5b65fjy7f3f.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:46Z",
      "native_id": "hal-05775817",
      "source": "hal",
      "url": "https://hal.science/hal-05775817v1"
    },
    {
      "first_seen": "2026-10-30T12:24:24Z",
      "native_id": "10.64898/2026.09.22.753382",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.22.753382v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:developmental biology",
      "subfield": "developmental-biology"
    }
  ],
  "title": "Transcriptional coupling of metabolism and cell mechanics controls human neuroepithelium morphogenesis",
  "title_text": "Transcriptional coupling of metabolism and cell mechanics controls human neuroepithelium morphogenesis",
  "type": "preprint",
  "updated_at": "2026-10-30T12:24:24Z",
  "url": "https://research.draconitic.com/paper/7e5b65fjy7f3f",
  "venue": "HAL",
  "versions": []
}