{
  "abstract": "Background\n\nDensity gradient sperm preparation may induce oxidative stress, impairing sperm function and deoxyribonucleic acid (DNA) integrity.Aim\n\nTo formulate and test an antioxidant-supplemented sperm wash with proteins.Settings and design\n\nAn in vitro pilot proof of concept with a paired sample design.Materials and methods\n\nTen normal and 10 abnormal semen samples were processed by double density gradient centrifugation of the sperm and then split into two portions that were placed in basal Ham's F10 and in an antioxidant-enriched medium (L-carnitine, N-acetyl cysteine and D-aspartic acid). Reactive oxygen species (ROS), motility, total motile sperm count (TMSC), morphology, teratozoospermic index (TZI) and DNA fragmentation were evaluated. High-performance liquid chromatography, nuclear magnetic resonance, molecular docking and molecular dynamics analyses were done.Statistical analyses used\n\nANOVA with Šidák/Tukey post hoc variations.Results\n\nROS (P<0.0001) and DNA fragmentation index (P< 0.001) levels were found to be significantly reduced with antioxidant supplementation. The motility with progressive movement and TMSC was still maintained (P< 0.01), especially in teratozoospermic specimens. Sperm recovery was similar for all media, and there were slight changes in morphology and in TZI. In silico results showed that binding to sperm function-associated protein targets was stable.Conclusion\n\nThe findings of this study indicate that antioxidant supplementation can decrease oxidative stress and improve sperm function during preparation. Studies with more subjects that include fertilisation, embryo development, pregnancy and live-birth outcomes are needed to validate.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Anatomy, Faculty of Medicine, Sri Ramachandra Institute of Higher Education and Research (DU), Chennai, Tamil Nadu, India.",
    "Crea Conceptions Pvt Ltd., Chennai, Tamil Nadu, India.",
    "Department of Anatomy, Annaii Medical College, Rajalakshmi Health City, Kanchipuram, Tamil Nadu, India.",
    "Sathyabama Institute of Science and Technology, Chennai, Tamil Nadu, India.",
    "Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education and Research (DU), Chennai, Tamil Nadu, India.",
    "Department of Biomedical Sciences, Faculty of Biomedical Sciences and Technology, Sri Ramachandra Institute of Higher Education and Research (DU), Chennai, Tamil Nadu, India.",
    "Department of Reproductive Medicine and Surgery, Sri Ramachandra Institute of Higher Education and Research (DU), Chennai, Tamil Nadu, India."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Sukanth Kumar Enmozhi"
    },
    {
      "affs": [
        1
      ],
      "name": "Sasikala Natarajamani"
    },
    {
      "affs": [
        2
      ],
      "name": "Gunapriya Raghunath"
    },
    {
      "affs": [
        3
      ],
      "name": "Jerrine Joseph"
    },
    {
      "affs": [
        4
      ],
      "name": "Infant Xavier"
    },
    {
      "affs": [
        4
      ],
      "name": "Theepan Raaj"
    },
    {
      "affs": [
        4
      ],
      "name": "S Pradeep"
    },
    {
      "affs": [
        4
      ],
      "name": "Niloy Raj Kalita"
    },
    {
      "affs": [
        5
      ],
      "name": "Sumathy Arockiasamy"
    },
    {
      "affs": [
        6
      ],
      "name": "Radha Vembu"
    },
    {
      "affs": [
        0
      ],
      "name": "Ananda Rani"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7emg90rcrakgr.bib",
  "cited_by": 0,
  "doi": "10.4103/jhrs.jhrs_32_26",
  "fulltext_url": "https://europepmc.org/articles/PMC13690310",
  "id": "7emg90rcrakgr",
  "issns": [
    "0974-1208",
    "1998-4766"
  ],
  "landing_url": "https://europepmc.org/article/MED/42923895",
  "language": "eng",
  "licence": "CC-BY-NC-SA",
  "licence_label": "CC BY-NC-SA",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-sa/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2743|Sperm and Testicular Function"
    ]
  ],
  "openalex": "W7214971556",
  "pdf_url": "https://europepmc.org/articles/PMC13690310?pdf=render",
  "pmcid": "PMC13690310",
  "pmid": "42923895",
  "published": "2026-07-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/7emg90rcrakgr.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:21:02Z",
      "native_id": "MED:42923895",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42923895"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2743|Sperm and Testicular Function",
      "subfield": "reproductive-child"
    }
  ],
  "title": "Evaluation of a Novel Antioxidant-supplemented Sperm Wash Medium on Human Sperm Function: A Pilot Study.",
  "title_text": "Evaluation of a Novel Antioxidant-supplemented Sperm Wash Medium on Human Sperm Function: A Pilot Study.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:21:02Z",
  "url": "https://research.draconitic.com/paper/7emg90rcrakgr",
  "venue": "Journal of human reproductive sciences",
  "versions": []
}