{
  "abstract": "Genetic loci influencing multiple complex traits often remain difficult to interpret because the underlying functional variants, target genes and tissue contexts are unresolved. Here, we investigated the regulatory basis of a shared signal for average daily gain (ADG) and backfat thickness (BF) in Yorkshire pigs. By integrating GWAS in 7,972 pigs, targeted capture sequencing, LD-based fine-mapping, porcine epigenomic annotations, three-dimensional chromatin interaction maps and functional assays, we narrowed the shared SSC1 signal to a 1.59-Mb interval and functionally prioritized rs334769214 (T>G) as the leading candidate variant. The T>G substitution produced marked allele-specific differences in enhancer activity and nuclear-protein binding, while transcription-factor co-transfection assays identified FOXL1-responsive, allele-dependent regulatory activity. Chromatin interaction and CRISPRa analyses supported a regulatory connection between the rs334769214-containing enhancer and PMAIP1, and PMAIP1 overexpression promoted apoptosis in porcine intestinal epithelial cells. In the capture-sequenced subset, the G allele was associated with higher ADG and BF, consistent with a relative loss-of-activation model. Together, these findings delineate a candidate FOXL1-enhancer-PMAIP1 regulatory pathway and demonstrate how multi-omics fine-mapping can advance a shared quantitative-trait locus toward a testable molecular mechanism underlying variation in growth and fatness.",
  "abstract_shown": true,
  "affiliations": [
    "Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Laboratory of Swine Genetics and Breeding of Ministry o"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "name": "H. Yang"
    },
    {
      "name": "M. Hu"
    },
    {
      "name": "Y. Xu"
    },
    {
      "name": "J. Zhang"
    },
    {
      "name": "Y. Zhang"
    },
    {
      "name": "Y. Gou"
    },
    {
      "name": "X. Du"
    },
    {
      "name": "H. Wang"
    },
    {
      "name": "X. Li"
    },
    {
      "name": "Y. Zhao"
    },
    {
      "affs": [
        0
      ],
      "name": "T. Xiang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7f1esed71jg55.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.26.754598",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.26.754598v1.full",
  "id": "7f1esed71jg55",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.26.754598v1",
  "language": null,
  "licence": "CC-BY-ND",
  "licence_label": "CC BY-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "genetics"
    ],
    [
      "openalex",
      "1311|Genetic and phenotypic traits in livestock"
    ]
  ],
  "openalex": "W7215118013",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.26.754598v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-26",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/7f1esed71jg55.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:25:42Z",
      "native_id": "10.64898/2026.09.26.754598",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.26.754598v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:genetics",
      "subfield": "genetics-genomics"
    }
  ],
  "title": "Multi-omics fine-mapping uncovers a pleiotropic intestinal enhancer variant linking PMAIP1 regulation to pig growth and fatness",
  "title_text": "Multi-omics fine-mapping uncovers a pleiotropic intestinal enhancer variant linking PMAIP1 regulation to pig growth and fatness",
  "type": "preprint",
  "updated_at": "2026-10-30T12:25:42Z",
  "url": "https://research.draconitic.com/paper/7f1esed71jg55",
  "venue": "bioRxiv",
  "versions": []
}