{
  "abstract": "Refractive index (RI)-matching-based tissue clearing techniques render intact biological tissues optically transparent, enabling 3D in situ interrogation of nervous systems from whole-brain to single-cell scales. We systematically evaluate three mainstream clearing strategies (hydrophobic, hydrophilic, and hydrogel-based) and summarize their promising applications in neural circuit tracing, tumor research, stem cell therapy, and artificial intelligence (AI) integration. Here, we report that this technology has evolved from a niche imaging tool to an integrated multimodal biotechnological platform. Despite core challenges, including inherent physicochemical trade-offs, optical limits, multi-omics integration biases, and insufficient clinical standardization, future advances in multimodal integration, AI algorithms, and targeted in vivo clearing will drive life science research from static structural mapping toward systematic dissection of dynamic biological processes.",
  "abstract_shown": true,
  "affiliations": [
    "Chinese Institute for Brain Research, Beijing, China",
    "Naval Medical University, No.800, Xiangyin Road, Shanghai 200433, China",
    "Department of Biomedical Sciences, Cancer Research and Specialist Centre, Universiti Sains Malaysia, Penang 13200, Malaysia",
    "Chinese Institute for Brain Research, Beijing, China; Corresponding author",
    "Naval Medical University, No.800, Xiangyin Road, Shanghai 200433, China; Corresponding author"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Man-Yu Chen"
    },
    {
      "affs": [
        1
      ],
      "name": "Xuan Yin"
    },
    {
      "affs": [
        2
      ],
      "name": "Haoling Zhang"
    },
    {
      "affs": [
        1
      ],
      "name": "Lizhou Song"
    },
    {
      "affs": [
        3
      ],
      "name": "Hu Zhao"
    },
    {
      "affs": [
        4
      ],
      "name": "Wangzheqi Zhang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7fh718qkwvqxk.bib",
  "cited_by": 0,
  "doi": "10.1016/j.xcrp.2026.103607",
  "fulltext_url": "http://www.sciencedirect.com/science/article/pii/S2666386426005138",
  "id": "7fh718qkwvqxk",
  "issns": [
    "2666-3864"
  ],
  "landing_url": "https://doi.org/10.1016/j.xcrp.2026.103607",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "QC1-999"
    ],
    [
      "openalex",
      "1304|Advanced Fluorescence Microscopy Techniques"
    ]
  ],
  "openalex": "W7223159133",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-30",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/7fh718qkwvqxk.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:43:44Z",
      "native_id": "aa18f4d04aec406980324ea7e156abbf",
      "source": "doaj",
      "url": "https://doi.org/10.1016/j.xcrp.2026.103607"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1304|Advanced Fluorescence Microscopy Techniques",
      "subfield": "biophysics"
    }
  ],
  "title": "Emerging and transformative applications of tissue clearing technology in neuroscience research",
  "title_text": "Emerging and transformative applications of tissue clearing technology in neuroscience research",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T13:43:44Z",
  "url": "https://research.draconitic.com/paper/7fh718qkwvqxk",
  "venue": "Cell Reports Physical Science",
  "versions": []
}