{
  "abstract": "Phytophthora infestans, the causal agent of late blight in potato and tomato, is a historically devastating pathogen that continues to pose a major threat to global food security. Although conventional fungicides remain a cornerstone of crop protection, growing concerns over their environmental impact and long‐term sustainability have driven the search for safer and more selective alternatives. In this context, next‐generation fungicides with high specificity and minimal ecological footprint are urgently needed. This study introduces CP32, a small cyclic peptide, identified via yeast two‐hybrid screening of a genetically encoded combinatorial peptide library, based on its specific interaction with the catalytic domain of the P. infestans enzyme Cellulose Synthase 2. CP32 exhibits anti‐oomycete activity, effectively inhibits sporangial growth at low micromolar concentrations, and significantly reduces late blight symptoms on tomato leaves and entire plants. In silico docking analyses, supported by molecular and biochemical evidence, confirm that CP32 compromises cell‐wall integrity by interfering with cellulose deposition and altering glucan composition. Furthermore, CP32 readily permeates the Phytophthora membrane and displays oomycete‐static properties, while showing no detectable toxicity toward non‐target organisms, such as bacteria, yeast, or plants. Collectively, these findings identify CP32 as a promising, targeted anti‐oomycete agent with a unique mode of action, targeting the P. infestans cell wall, representing a valuable contribution to the development of sustainable strategies for the control of late blight.",
  "abstract_shown": true,
  "affiliations": [
    "Dipartimento di Bioscienze, Università degli Studi di Milano Milano 20133 Italy",
    "Division of Glycoscience, Department of Chemistry, School of Engineering Sciences in Chemistry, Biotechnology and Health Royal Institute of Technology (KTH) Stockholm SE 106 91 Sweden",
    "Dipartimento di Scienze Farmaceutiche Università degli Studi di Milano Milano 20133 Italy",
    "Dipartimento di Fisiopatologia Medico‐Chirurgica e dei Trapianti Università degli Studi di Milano Milan 20100 Italy",
    "College of Medicine and Public Health Flinders University, Bedford Park Campus Adelaide 5042 South Australia Australia",
    "Molecular Biology and Nanotechnology Laboratory (MolBNL@UniTS), DEA University of Trieste Trieste 34127 Italy",
    "Department of General Biophysics, Faculty of Biology and Environmental Protection University of Lodz Łódź 90‐236 Poland",
    "Université Paris‐Saclay, CNRS, BioCIS Orsay 91400 France",
    "Dipartimento di Scienze Agrarie e Ambientali—Produzione, Territorio e Agroenergia Università degli Studi di Milano Milano 20133 Italy"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 24,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Demetrio Marcianò",
      "orcid": "0000-0003-4781-9593"
    },
    {
      "affs": [
        0
      ],
      "name": "Stefano Rosa",
      "orcid": "0000-0003-0856-6351"
    },
    {
      "affs": [
        0
      ],
      "name": "Elena Marone Fassolo",
      "orcid": "0000-0002-6646-898X"
    },
    {
      "affs": [
        0
      ],
      "name": "Chiara Bertaso",
      "orcid": "0009-0000-9771-3274"
    },
    {
      "affs": [
        0
      ],
      "name": "Andrea Tagliani",
      "orcid": "0000-0002-8718-5209"
    },
    {
      "affs": [
        0
      ],
      "name": "Nina Gauri Capra",
      "orcid": "0009-0006-7439-4649"
    },
    {
      "affs": [
        0
      ],
      "name": "Federico Ballabio",
      "orcid": "0000-0001-5702-3674"
    },
    {
      "affs": [
        0
      ],
      "name": "Chiara Mizzotti",
      "orcid": "0000-0003-3644-2890"
    },
    {
      "affs": [
        0
      ],
      "name": "Giulia Rizzi",
      "orcid": "0000-0002-0635-4729"
    },
    {
      "affs": [
        1
      ],
      "name": "Sadia Fida Ullah",
      "orcid": "0000-0002-2052-9214"
    },
    {
      "affs": [
        1
      ],
      "name": "Lisa Kappel",
      "orcid": "0000-0001-9633-9030"
    },
    {
      "affs": [
        2
      ],
      "name": "Lucia Feni",
      "orcid": "0009-0006-8497-4938"
    },
    {
      "affs": [
        0
      ],
      "name": "Luca Tadini",
      "orcid": "0000-0001-7446-325X"
    },
    {
      "affs": [
        0
      ],
      "name": "Louise Jane Gourlay",
      "orcid": "0000-0002-6369-2230"
    },
    {
      "affs": [
        3
      ],
      "name": "Rosaria Russo",
      "orcid": "0000-0003-0011-5775"
    },
    {
      "affs": [
        4
      ],
      "name": "Vincent Bulone",
      "orcid": "0000-0003-2809-4160"
    },
    {
      "affs": [
        5,
        6
      ],
      "name": "Sabrina Pricl",
      "orcid": "0000-0001-8380-4474"
    },
    {
      "affs": [
        7
      ],
      "name": "Sandrine Ongeri",
      "orcid": "0000-0002-2118-7324"
    },
    {
      "affs": [
        2
      ],
      "name": "Sara Pellegrino",
      "orcid": "0000-0002-2325-3583"
    },
    {
      "affs": [
        0
      ],
      "name": "Carlo Camilloni",
      "orcid": "0000-0002-9923-8590"
    },
    {
      "affs": [
        8
      ],
      "name": "Silvia Laura Toffolatti",
      "orcid": "0000-0003-3572-8686"
    },
    {
      "affs": [
        1
      ],
      "name": "Vaibhav Srivastava",
      "orcid": "0000-0003-1877-4154"
    },
    {
      "affs": [
        0
      ],
      "name": "Paolo Pesaresi",
      "orcid": "0000-0002-3236-7005"
    },
    {
      "affs": [
        0
      ],
      "name": "Simona Masiero",
      "orcid": "0000-0002-7563-7634"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7ftc41s2ne2bk.bib",
  "cited_by": 0,
  "doi": "10.1111/jipb.70343",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13725601/",
  "id": "7ftc41s2ne2bk",
  "issns": [
    "1672-9072",
    "1744-7909"
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  "landing_url": "https://doi.org/10.1111/jipb.70343",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Plant Biotic Interactions"
    ],
    [
      "heading",
      "Research Article"
    ],
    [
      "openalex",
      "1110|Plant Pathogens and Resistance"
    ]
  ],
  "openalex": "W7168923105",
  "pdf_url": null,
  "pmcid": "PMC13725601",
  "pmid": "42461145",
  "published": "2026-07-16",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/7ftc41s2ne2bk.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T12:23:57Z",
      "native_id": "PMC13725601",
      "source": "pmc",
      "url": "https://doi.org/10.1111/jipb.70343"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1110|Plant Pathogens and Resistance",
      "subfield": "plant-biology"
    }
  ],
  "title": "CP32, a novel anti‐oomycete cyclic peptide, interferes with cell‐wall deposition in Phytophthora infestans",
  "title_text": "CP32, a novel anti‐oomycete cyclic peptide, interferes with cell‐wall deposition in Phytophthora infestans",
  "type": "peer-reviewed",
  "updated_at": "2026-11-05T12:22:09Z",
  "url": "https://research.draconitic.com/paper/7ftc41s2ne2bk",
  "venue": "Journal of Integrative Plant Biology",
  "versions": []
}