{
  "abstract": "(1) Background: Acetamiprid, a widely used neonicotinoid insecticide, has increasingly drawn people's attention to its potential reproductive toxicity risks. Although existing studies have shown that exposure to acetamiprid can cause testicular damage, its underlying molecular mechanism has not been fully elucidated. (2) Methods: This study established a mouse model of acetamiprid exposure via free drinking water and combined it with in vitro culture of TM3 cells, aiming to explore the toxic effects of acetamiprid on testicular tissue and its potential mechanism. (3) Results: Acetamiprid exposure induced significant pathological damage to the testicular tissue of mice and significantly reduced sperm quality. Further mechanism studies have shown that acetamiprid down-regulates the expression of RNA demethylase ALKBH5, leading to an overall increase in m⁶A modification levels, thereby inhibiting the activity of the PI3K/AKT signaling pathway and ultimately inducing apoptosis of TM3 cells. (4) Conclusions: In conclusion, this study, by integrating both internal and external models, has revealed a molecular mechanism by which acetamiprid is involved in testicular injury through epigenetic pathways, providing scientific basis and mechanism clues for assessing reproductive hazards caused by exposure to environmental toxins.",
  "abstract_shown": true,
  "affiliations": [
    "Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Medical University, Yinchuan 750004, China.",
    "Key Laboratory of Environmental Factors and Chronic Disease Control, College of Public Health, Ningxia Medical University, Yinchuan 750004, China.",
    "Department of Toxicology, Shaanxi Provincial Key Lab of Free Radical Biology and Medicine, School of Public Health, Fourth Military Medical University, Xi'an 710032, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Xiaoyan Hua"
    },
    {
      "affs": [
        1
      ],
      "name": "Yubin Li"
    },
    {
      "affs": [
        1
      ],
      "name": "Lixiang Pang"
    },
    {
      "affs": [
        1
      ],
      "name": "Huiyuan Zhang"
    },
    {
      "affs": [
        1
      ],
      "name": "Yuwen Wang"
    },
    {
      "affs": [
        1
      ],
      "name": "Bing Wu"
    },
    {
      "affs": [
        1
      ],
      "name": "Haoxuan Gao",
      "orcid": "0000-0002-3368-4387"
    },
    {
      "affs": [
        1
      ],
      "name": "Yanan Song",
      "orcid": "0000-0003-2504-5515"
    },
    {
      "affs": [
        1
      ],
      "name": "Yucheng Yan"
    },
    {
      "affs": [
        1
      ],
      "name": "Huifang Yang"
    },
    {
      "affs": [
        0
      ],
      "name": "Yikai Qiu"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Ji Zhao"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7ftxjjda4e7dt.bib",
  "cited_by": 0,
  "doi": "10.3390/toxics14100903",
  "fulltext_url": "https://europepmc.org/articles/PMC13709516",
  "id": "7ftxjjda4e7dt",
  "issns": [
    "2305-6304"
  ],
  "landing_url": "https://europepmc.org/article/MED/42931690",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2743|Sperm and Testicular Function"
    ]
  ],
  "openalex": "W7221507191",
  "pdf_url": "https://europepmc.org/articles/PMC13709516?pdf=render",
  "pmcid": "PMC13709516",
  "pmid": "42931690",
  "published": "2026-10-12",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/7ftxjjda4e7dt.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:01Z",
      "native_id": "MED:42931690",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42931690"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2743|Sperm and Testicular Function",
      "subfield": "reproductive-child"
    }
  ],
  "title": "The Toxic Effects and Mechanism of Acetamiprid Exposure on Testicular Tissue: ALKBH5 Regulates m⁶A Modification to Affect PI3K-AKT-Mediated Apoptosis.",
  "title_text": "The Toxic Effects and Mechanism of Acetamiprid Exposure on Testicular Tissue: ALKBH5 Regulates m⁶A Modification to Affect PI3K-AKT-Mediated Apoptosis.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:22:01Z",
  "url": "https://research.draconitic.com/paper/7ftxjjda4e7dt",
  "venue": "Toxics",
  "versions": []
}