{
  "abstract": "Pachymic acid (PA), a major bioactive component of Poria cocos, suppresses colorectal cancer (CRC) proliferation and invasion by disrupting glycolysis. However, its antitumor activity and underlying mechanisms in vitro and in vivo remain unclear. RNA-binding proteins (RBPs) are key post-transcriptional regulators in CRC, but their mechanisms of action and therapeutic potential remain poorly understood. Here, we employed limited proteolysis-mass spectrometry (LiP-MS), a target deconvolution approach that does not require prior chemical modification, to profile changes in protein conformation within complex proteomes upon PA treatment in cells. Our findings revealed that RBMX (RNA-binding motif protein X-linked) acts as a target of PA and undergoes concentration-dependent conformational changes, as supported by binding validation and functional assays performed in vitro and in vivo. Furthermore, proximity-labeling mass spectrometry (PL-MS) analysis revealed that RBMX interacts with heterogeneous nuclear ribonucleoprotein C (hnRNPC). Functional assays demonstrated that PA treatment induces RBMX-dependent degradation of hnRNPC, thereby suppressing CRC cell proliferation and tumor growth. In summary, this study identifies RBMX as a molecular target of PA through LiP-MS, providing new insights into the mechanisms underlying PA-mediated CRC suppression and highlighting the RBMX–hnRNPC axis as an important mediator of its antitumor effects.",
  "abstract_shown": true,
  "affiliations": [
    "School of Pharmaceutical Sciences, State Key Laboratory of Traditional Chinese Medicine Syndrome, Chinese Medicine Guangdong Laboratory (Hengqin), Guangzhou University of Chinese Medicine, Guangzhou 510006, China",
    "Pingshan Translational Medicine Center, Shenzhen Bay Laboratory, Shenzhen 518118, China",
    "Shenzhen Bay Laboratory, Shenzhen 518132, China",
    "State Key Laboratory of Chemical Oncogenomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen 518055, China",
    "Laboratory of Advanced Theranostic Materials and Technology, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315300, China",
    "Ningbo Cixi Institute of Biomedical Engineering, Ningbo 315300, China"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Weichao Ling"
    },
    {
      "affs": [
        0
      ],
      "name": "Meijing Song"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Xinlin Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Yu Li"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Yuhao An"
    },
    {
      "affs": [
        1,
        2,
        3
      ],
      "name": "Zhidong Chen"
    },
    {
      "affs": [
        1
      ],
      "name": "Zhijun Ruan"
    },
    {
      "affs": [
        4,
        5
      ],
      "name": "Yang Zhou",
      "orcid": "0000-0002-2023-0941"
    },
    {
      "affs": [
        0
      ],
      "name": "Linlin Lu"
    },
    {
      "affs": [
        1,
        2,
        3
      ],
      "name": "Zigang Li"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Rui Wang",
      "orcid": "0000-0003-3830-4133"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7k820rvf1nkh7.bib",
  "cited_by": 0,
  "doi": "10.3390/cells15211992",
  "fulltext_url": null,
  "id": "7k820rvf1nkh7",
  "issns": [
    "2073-4409"
  ],
  "landing_url": "https://www.mdpi.com/2073-4409/15/21/1992",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-31",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/7k820rvf1nkh7.ris",
  "sources": [
    {
      "first_seen": "2026-11-03T00:24:04Z",
      "native_id": "10.3390/cells15211992",
      "source": "crossref",
      "url": "https://www.mdpi.com/2073-4409/15/21/1992"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "issn:2073-4409",
      "subfield": "cell-biology"
    }
  ],
  "title": "Comprehensive Proteomics Reveals Pachymic Acid Targeting RBMX via hnRNPC Degradation in Colorectal Cancer",
  "title_text": "Comprehensive Proteomics Reveals Pachymic Acid Targeting RBMX via hnRNPC Degradation in Colorectal Cancer",
  "type": "peer-reviewed",
  "updated_at": "2026-11-04T00:24:56Z",
  "url": "https://research.draconitic.com/paper/7k820rvf1nkh7",
  "venue": "Cells",
  "versions": []
}