{
  "abstract": "Breast cancer remains a major clinical challenge because of its molecular heterogeneity, therapeutic resistance, and disease recurrence. Melittin, the principal bioactive peptide of honeybee venom, exhibits antitumor activity in multiple experimental models; however, its early proteome-wide effects in breast cancer cells remain incompletely characterized. In this study, E0771 murine mammary carcinoma cells were exposed to melittin for 15 min. Colony formation and CCK-8 assays using 0-8 μg/mL melittin showed concentration-dependent reductions in clonogenic capacity and cell viability. For DIA-based quantitative proteomic profiling, E0771 cells were treated with 4 μg/mL melittin for 15 min. Among 9748 quantified protein entries, 339 proteins met the exploratory criteria for candidate differential abundance, including 114 up-regulated and 225 down-regulated candidates. GO and KEGG analyses associated these candidate proteins with cell junctions, cytoskeletal organization, protein kinase-related functions, metabolic processes, and cell-death-associated pathways. A STRING-derived association network containing 132 nodes and 216 edges further prioritized CASP8, RPS27A, ANXA5, and PIK3CA as relatively high-connectivity candidates. Collectively, these findings describe early proteomic responses to short-term melittin exposure and provide candidate proteins and biological processes for subsequent mechanistic validation rather than establishing causal molecular mechanisms.",
  "abstract_shown": true,
  "affiliations": [
    "Ningbo No. 2 Hospital, Wenzhou Medical University, Ningbo 315000, China.",
    "National & Local United Engineering Laboratory of Natural Biotoxin, Fuzhou 350002, China.",
    "Apitherapy Research Institute, Fujian Agriculture and Forestry University, Fuzhou 350002, China.",
    "College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Junling Zhu"
    },
    {
      "affs": [
        1,
        2,
        3
      ],
      "name": "Wenhua Xu"
    },
    {
      "affs": [
        1,
        2,
        3
      ],
      "name": "He Zang"
    },
    {
      "affs": [
        1,
        2,
        3
      ],
      "name": "Yunzhen Yang"
    },
    {
      "affs": [
        1,
        2,
        3
      ],
      "name": "Nian Fan"
    },
    {
      "affs": [
        1,
        2,
        3
      ],
      "name": "Yuwei Zhang"
    },
    {
      "affs": [
        1,
        2,
        3
      ],
      "name": "Yaqin Gao"
    },
    {
      "affs": [
        1,
        2,
        3
      ],
      "name": "Jianfeng Qiu",
      "orcid": "0000-0002-4262-9013"
    },
    {
      "affs": [
        1,
        2,
        3
      ],
      "name": "Dafu Chen"
    },
    {
      "affs": [
        1,
        2,
        3
      ],
      "name": "Rui Guo"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7kfk1xdhr28vg.bib",
  "cited_by": 0,
  "doi": "10.3390/biology15191727",
  "fulltext_url": "https://europepmc.org/articles/PMC13663608",
  "id": "7kfk1xdhr28vg",
  "issns": [
    "2079-7737"
  ],
  "landing_url": "https://europepmc.org/article/MED/42883400",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2736|Healthcare and Venom Research"
    ]
  ],
  "openalex": "W7215042247",
  "pdf_url": "https://europepmc.org/articles/PMC13663608?pdf=render",
  "pmcid": "PMC13663608",
  "pmid": "42883400",
  "published": "2026-10-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/7kfk1xdhr28vg.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:00Z",
      "native_id": "MED:42883400",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42883400"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2736|Healthcare and Venom Research",
      "subfield": "pharmacology-toxicology"
    }
  ],
  "title": "DIA Quantitative Proteomic Analysis Reveals Early Proteomic Responses to Honeybee-Derived Melittin in Murine Breast Cancer Cells.",
  "title_text": "DIA Quantitative Proteomic Analysis Reveals Early Proteomic Responses to Honeybee-Derived Melittin in Murine Breast Cancer Cells.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:00Z",
  "url": "https://research.draconitic.com/paper/7kfk1xdhr28vg",
  "venue": "Biology",
  "versions": []
}