{
  "abstract": "An in vitro model of human liver with functional integrity would advance liver research and enable personalized therapeutics. A microfluidic liver-on-a-chip can generate a functional hepatobiliary network using human liver cells and recapitulate blood circulation and bile excretion. However, many current microfluidic liver models lack a biliary component and cannot fully recapitulate the excretory function of the human liver. Here, we develop a microfluidic liver-on-a-chip incorporating functional endothelial cells, hepatocytes, and cholangiocytes derived from a single source of human mesenchymal stem cells. This stem cell-derived liver-on-a-chip reconstructs a simplified liver unit, including a sinusoid, a hepatocyte compartment, and a bile duct supported by barrier integrity and fluid flow. It reproduces integrated organ-level responses to xenobiotics, including hepatic uptake and biliary excretion, and models ischemic liver injury with therapeutic cell recruitment. This technology expands personalized organ-level modeling and holds strong potential for studying tissue development and advancing targeted therapies for liver injury.",
  "abstract_shown": true,
  "affiliations": [
    "Frazer Institute, Faculty of Health, Medicine and Behavioural Science, The University of Queensland, Brisbane, QLD 4102, Australia",
    "Gallipoli Medical Research, Greenslopes Private Hospital, Brisbane, QLD 4120, Australia",
    "Faculty of Health, Medicine and Behavioural Science, The University of Queensland, Brisbane, QLD 4006, Australia",
    "School of Chemical Engineering and Advanced Materials, The University of Adelaide, Adelaide, SA 5005, Australia",
    "School of Biomedical Science, Faculty of Health, Queensland University of Technology (QUT), Brisbane, QLD 4000, Australia"
  ],
  "article_type": "brief report",
  "arxiv": null,
  "author_total": 15,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Anastasia Brooks"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Haotian Yang"
    },
    {
      "affs": [
        0
      ],
      "name": "Deborah S. Barkauskas"
    },
    {
      "affs": [
        3
      ],
      "name": "Supun Ranaweera"
    },
    {
      "affs": [
        3
      ],
      "name": "Da Zou"
    },
    {
      "affs": [
        0
      ],
      "name": "Sally Goh"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Qi Ruan"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Kim Bridle"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Darrell H.G. Crawford"
    },
    {
      "affs": [
        0
      ],
      "name": "Xin Liu"
    },
    {
      "affs": [
        0
      ],
      "name": "Michael S. Roberts"
    },
    {
      "affs": [
        4
      ],
      "name": "Michael R. Doran"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Xiaowen Liang"
    },
    {
      "affs": [
        3
      ],
      "name": "Chun-Xia Zhao"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Haolu Wang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7kr35dwdq7728.bib",
  "cited_by": 0,
  "doi": "10.1016/j.crmeth.2026.101545",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13721747/",
  "id": "7kr35dwdq7728",
  "issns": [
    "2667-2375"
  ],
  "landing_url": "https://doi.org/10.1016/j.crmeth.2026.101545",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Report"
    ],
    [
      "openalex",
      "2204|3D Printing in Biomedical Research"
    ]
  ],
  "openalex": "W7202080746",
  "pdf_url": null,
  "pmcid": "PMC13721747",
  "pmid": "42575102",
  "published": "2026-08-10",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/7kr35dwdq7728.ris",
  "sources": [
    {
      "first_seen": "2026-11-03T12:22:23Z",
      "native_id": "PMC13721747",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.crmeth.2026.101545"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "journal-openalex:1312|Single-cell and spatial transcriptomics",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Mesenchymal stem cell-derived liver-on-a-chip reconstitutes human liver structure and function",
  "title_text": "Mesenchymal stem cell-derived liver-on-a-chip reconstitutes human liver structure and function",
  "type": "peer-reviewed",
  "updated_at": "2026-11-03T12:22:23Z",
  "url": "https://research.draconitic.com/paper/7kr35dwdq7728",
  "venue": "Cell Reports Methods",
  "versions": []
}