{
  "abstract": "Efficient systemic delivery to the lung remains a major barrier for adeno-associated virus (AAV)-mediated pulmonary gene therapy, particularly when pre-existing immunity limits the use of conventional capsids. Here, we evaluated Bovine AAV, a phylogenetically divergent capsid, as a candidate vector for lung-directed gene transfer. In adult C57BL/6J mice, intravenous delivery of Bovine AAV resulted in robust and preferential lung transduction comparable with AAV4, with predominant targeting of alveolar type I pneumocytes and pulmonary endothelial cells. In primary human lung-resident cells, Bovine AAV was particularly effective in microvascular endothelial cells, a target poorly transduced by AAV4 in vitro. Bovine AAV demonstrated scalable production with yield, purification performance, capsid quality, and genome integrity comparable to AAV9. In sera from healthy adults from the United States and Switzerland, Bovine AAV showed intermediate neutralization frequencies, lower than AAV2 and AAV4 but higher than AAV5 and AAV9. Of relevance, Bovine AAV maintained in vivo transduction efficiency in mice previously immunized with a pool of human and non-human primate-derived AAV capsids, including AAV4. Together, these results position Bovine AAV as a promising lung-tropic and immune-distinct vector for pulmonary gene therapy, with particular relevance for applications requiring systemic delivery in the presence of pre-existing immunity to conventional serotypes.",
  "abstract_shown": true,
  "affiliations": [
    "Novartis Biomedical Research, Basel 4056, Switzerland",
    "Novartis Biomedical Research, San Diego, CA 92121, USA",
    "Novartis Biomedical Research, Cambridge, MA 02139, USA",
    "University of Basel, Faculty of Science, Department of Pharmaceutical Sciences, Basel 4056, Switzerland"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 25,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Daniela C. Ivan"
    },
    {
      "affs": [
        0
      ],
      "name": "Valerie Dubost"
    },
    {
      "affs": [
        0
      ],
      "name": "Laura Israel"
    },
    {
      "affs": [
        0
      ],
      "name": "Jonas Weinmann"
    },
    {
      "affs": [
        0
      ],
      "name": "Dennis Ungan"
    },
    {
      "affs": [
        0
      ],
      "name": "Nina Carbonetti"
    },
    {
      "affs": [
        0
      ],
      "name": "Nathalie Stuber"
    },
    {
      "affs": [
        0
      ],
      "name": "Magali Jivkov"
    },
    {
      "affs": [
        0
      ],
      "name": "Esther Erard"
    },
    {
      "affs": [
        0
      ],
      "name": "Elisabetta Biglieri"
    },
    {
      "affs": [
        0
      ],
      "name": "Federica De Girardi"
    },
    {
      "affs": [
        0
      ],
      "name": "Simon Mittermeier"
    },
    {
      "affs": [
        0
      ],
      "name": "Maryam Syed"
    },
    {
      "affs": [
        0
      ],
      "name": "Bruno Tigani"
    },
    {
      "affs": [
        0
      ],
      "name": "Najwa Ouali Alami"
    },
    {
      "affs": [
        0
      ],
      "name": "Kristin Dreessen"
    },
    {
      "affs": [
        1
      ],
      "name": "Colin Deniston"
    },
    {
      "affs": [
        2
      ],
      "name": "Kannan Sankar"
    },
    {
      "affs": [
        0
      ],
      "name": "Laura Bollepalli"
    },
    {
      "affs": [
        0
      ],
      "name": "Vanessa Cornacchione"
    },
    {
      "affs": [
        0
      ],
      "name": "Elisabetta Traggiai"
    },
    {
      "affs": [
        0
      ],
      "name": "Dominique Brees"
    },
    {
      "affs": [
        0
      ],
      "name": "Anette Karle"
    },
    {
      "affs": [
        0,
        3
      ],
      "name": "José M. Carballido"
    },
    {
      "affs": [
        0
      ],
      "name": "Agostino Cirillo"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7ksxqt3t1ymy1.bib",
  "cited_by": 0,
  "doi": "10.1016/j.omta.2026.201859",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13734831/",
  "id": "7ksxqt3t1ymy1",
  "issns": [
    "3117-387X"
  ],
  "landing_url": "https://doi.org/10.1016/j.omta.2026.201859",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Original Article"
    ],
    [
      "openalex",
      "1311|Virus-based gene therapy research"
    ]
  ],
  "openalex": "W7220470749",
  "pdf_url": null,
  "pmcid": "PMC13734831",
  "pmid": "42990936",
  "published": "2026-10-05",
  "publisher": "American Society of Gene & Cell Therapy",
  "ris": "https://research.draconitic.com/paper/7ksxqt3t1ymy1.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T12:20:39Z",
      "native_id": "PMC13734831",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.omta.2026.201859"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1311|Virus-based gene therapy research",
      "subfield": "genetics-genomics"
    }
  ],
  "title": "Bovine AAV: A promising vector for pulmonary gene therapy",
  "title_text": "Bovine AAV: A promising vector for pulmonary gene therapy",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T12:20:39Z",
  "url": "https://research.draconitic.com/paper/7ksxqt3t1ymy1",
  "venue": "Molecular Therapy Advances",
  "versions": []
}