{
  "abstract": "Introduction: Plasma phosphorylated tau 217 (p-tau217) and amyloid-{beta} 42 (A{beta}42) are advancing toward clinical use for Alzheimer's disease (AD), but their reliability can be impacted by preanalytical handling. Although alternative collection tubes with distinct stabilization strategies --P100 (protease inhibitor) and HEMAcollect (hemolysis/platelet-activation minimization) --have been developed to stabilize plasma proteins, how well they preserve these markers remains unresolved; we therefore compared both against fresh standard EDTA tubes. Methods: In 40 participants, plasma p-tau217 and A{beta}42 were measured (Lumipulse) in paired fresh EDTA, P100, and HEMAcollect samples after 5-day ambient storage. We assessed concentration change, rank-order preservation (Spearman {rho}), percentage difference, and diagnostic classification under the p-tau217/A{beta}42 ratio and p-tau217 alone. Results: P100 preserved p-tau217 favorably, with high rank-order correlation ({rho}=0.98) and a modest median change (+12.1%), and its classification agreement with fresh EDTA reached 97.5% using p-tau217 alone. HEMAcollect showed greater p-tau217 signal loss (-57.5%). A{beta}42 was reduced in both tubes (-72.8% P100; -95.5% HEMAcollect) with loss of rank order, so neither preserved the p-tau217/A{beta}42 ratio. Discussion: Stability was tube- and analyte-specific, and P100 consistently outperformed HEMAcollect --preserving p-tau217 rank order while HEMAcollect reduced signal across analytes. Although neither tube preserved the A{beta}42-dependent ratio, P100 shows potential for p-tau217-based workflows. Preanalytical stability must be established analyte by analyte before alternative tubes enter clinical AD diagnosis.",
  "abstract_shown": true,
  "affiliations": [
    "University of Pittsburgh"
  ],
  "article_type": "PUBLISHAHEADOFPRINT",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "name": "Y.-R. Lee"
    },
    {
      "name": "A. Raj"
    },
    {
      "name": "O. Lopez"
    },
    {
      "name": "X. Zeng"
    },
    {
      "affs": [
        0
      ],
      "name": "T. K. Karikari"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7m824n9d84hq8.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.30.26366958",
  "fulltext_url": "https://www.medrxiv.org/content/10.64898/2026.10.30.26366958v1.full",
  "id": "7m824n9d84hq8",
  "issns": [],
  "landing_url": "https://www.medrxiv.org/content/10.64898/2026.10.30.26366958v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "medrxiv",
      "psychiatry and clinical psychology"
    ]
  ],
  "openalex": null,
  "pdf_url": "https://www.medrxiv.org/content/10.64898/2026.10.30.26366958v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-30",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/7m824n9d84hq8.ris",
  "sources": [
    {
      "first_seen": "2026-11-03T00:20:25Z",
      "native_id": "10.64898/2026.10.30.26366958",
      "source": "medrxiv",
      "url": "https://www.medrxiv.org/content/10.64898/2026.10.30.26366958v1"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "medrxiv:psychiatry and clinical psychology",
      "subfield": "psychiatry"
    }
  ],
  "title": "Delayed-centrifugation blood collection tube for remote blood biomarker assessment",
  "title_text": "Delayed-centrifugation blood collection tube for remote blood biomarker assessment",
  "type": "preprint",
  "updated_at": "2026-11-04T00:21:00Z",
  "url": "https://research.draconitic.com/paper/7m824n9d84hq8",
  "venue": "medRxiv",
  "versions": []
}