{
  "abstract": "Benign prostatic hyperplasia (BPH) is common in aging men, but the endothelial compartment in enlarged prostates remains poorly defined. In this hypothesis-generating pilot study, we generated a single-cell RNA-seq dataset from six surgically obtained BPH specimens (three Large BPH and three Small BPH) and analyzed 56,912 cells; public datasets GSE290213 and GSE242249 were used for external context. We observed a higher sample-level fraction of Activated endothelial cells and a lower fraction of Venous-like endothelial cells in Large BPH, but these descriptive summaries were not designed for formal group inference. RNA velocity, graph ordering, and focused Slingshot/tradeSeq analyses nominated a putative endothelial-state ordering; concordance between graph pseudotime and latent time was modest (Spearman rho = 0.252). Variance decomposition suggested that AP-1/NF-κB activity was largely linked to endothelial-state composition, whereas selected IFN/HLA-II-associated programs showed stronger group-linked genetic context. MAGMA and TWAS did not support Activated endothelial markers or Large-up endothelial genes as the main genetic signal; broader stromal-vascular and HLA-related patterns were more consistent with the genetic data. These findings nominate endothelial activation signatures and a two-axis exploratory model for future validation in larger clinically annotated cohorts with protein-level testing.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Urology, Guangzhou Liwan Central Hospital",
    "Department of Urology, Zhujiang Hospital, Southern Medical University"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Wei Gao"
    },
    {
      "affs": [
        0
      ],
      "name": "Guoshang Yang"
    },
    {
      "affs": [
        1
      ],
      "name": "Chunxiao Chen"
    },
    {
      "affs": [
        0
      ],
      "name": "Jiajun Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Jing Lu"
    },
    {
      "affs": [
        0
      ],
      "name": "Mingkun Hu"
    },
    {
      "affs": [
        0
      ],
      "name": "Shengjun Liang"
    },
    {
      "affs": [
        0
      ],
      "name": "Changchun Xie"
    },
    {
      "affs": [
        1
      ],
      "name": "Ning Jiang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7nvmsgk1xc7pa.bib",
  "cited_by": 0,
  "doi": "10.1038/s41598-026-63751-1",
  "fulltext_url": "https://doi.org/10.1038/s41598-026-63751-1",
  "id": "7nvmsgk1xc7pa",
  "issns": [
    "2045-2322"
  ],
  "landing_url": "https://doi.org/10.1038/s41598-026-63751-1",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "Q"
    ],
    [
      "lcc",
      "R"
    ],
    [
      "openalex",
      "2748|Urinary Bladder and Prostate Research"
    ]
  ],
  "openalex": "W7170164755",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-07-01",
  "publisher": "Nature Portfolio",
  "ris": "https://research.draconitic.com/paper/7nvmsgk1xc7pa.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:45:54Z",
      "native_id": "d0ea9eeebada4ee5b2ff241420c8f85d",
      "source": "doaj",
      "url": "https://doi.org/10.1038/s41598-026-63751-1"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2748|Urinary Bladder and Prostate Research",
      "subfield": "general"
    }
  ],
  "title": "A pilot single-cell RNA sequencing study of endothelial activation signatures in large benign prostatic hyperplasia",
  "title_text": "A pilot single-cell RNA sequencing study of endothelial activation signatures in large benign prostatic hyperplasia",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T13:45:54Z",
  "url": "https://research.draconitic.com/paper/7nvmsgk1xc7pa",
  "venue": "Scientific Reports",
  "versions": []
}