{
  "abstract": "Drought adaptation requires rapid spatial reorganization of signaling proteins to reprogram stress-responsive transcription, yet how phosphorylation coordinates this process remains unclear. Here we aimed to define how phosphorylation-gated protein relocalization regulates a BpLBD1-centered drought pathway in Betula platyphylla (birch). Drought activated two kinase branches: BpAGC phosphorylated BpMAPK6 at Ser318 within a cryptic nuclear localization signal, promoting importin α recognition, whereas BpCKII phosphorylated BpGAPDH at Thr109, Thr270, Ser279 and Thr298, promoting importin β recognition. Dual phosphorylation enabled coordinated nuclear accumulation of BpMAPK6 and BpGAPDH. In the nucleus, phosphorylated BpGAPDH acted as a scaffold-like adaptor coupling BpMAPK6 to BpLBD1, enabling BpMAPK6-mediated phosphorylation of BpLBD1 at Ser29. This weakened BpLBD1 binding to the BpMYB123 promoter, reduced expression of the drought-sensitivity regulator BpMYB123, and de-repressed drought-responsive genes such as BpRD29B. Disrupting BpGAPDH or BpLBD1 Ser29 phosphorylation compromised drought tolerance. Thus, a phospho-spatial GAPDH-MAPK6-LBD1 switch couples nuclear protein assembly to drought-responsive transcription.",
  "abstract_shown": true,
  "affiliations": [
    "State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China.",
    "Shenyang Agricultural University, Shenyang, 110866, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Zihang He",
      "orcid": "0000-0002-3076-6299"
    },
    {
      "affs": [
        0
      ],
      "name": "Zhibo Wang",
      "orcid": "0000-0002-8733-5860"
    },
    {
      "affs": [
        0
      ],
      "name": "Caiqiu Gao",
      "orcid": "0000-0001-9953-9775"
    },
    {
      "affs": [
        1
      ],
      "name": "Xiaoyu Ji",
      "orcid": "0000-0001-9875-0675"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Yucheng Wang",
      "orcid": "0000-0003-3943-8811"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7q9f2g11pcep1.bib",
  "cited_by": 0,
  "doi": "10.1126/sciadv.aek0125",
  "fulltext_url": "https://europepmc.org/articles/PMC13685915",
  "id": "7q9f2g11pcep1",
  "issns": [
    "2375-2548"
  ],
  "landing_url": "https://www.science.org/doi/10.1126/sciadv.aek0125",
  "language": "eng",
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1110|Plant Stress Responses and Tolerance"
    ]
  ],
  "openalex": "W7223091706",
  "pdf_url": "https://europepmc.org/articles/PMC13685915?pdf=render",
  "pmcid": "PMC13685915",
  "pmid": "42905831",
  "published": "2026-10-21",
  "publisher": "American Association for the Advancement of Science (AAAS)",
  "ris": "https://research.draconitic.com/paper/7q9f2g11pcep1.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:41Z",
      "native_id": "10.1126/sciadv.aek0125",
      "source": "crossref",
      "url": "https://www.science.org/doi/10.1126/sciadv.aek0125"
    },
    {
      "first_seen": "2026-10-30T12:19:53Z",
      "native_id": "MED:42905831",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42905831"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1110|Plant Stress Responses and Tolerance",
      "subfield": "plant-biology"
    }
  ],
  "title": "A phosphorylation-gated GAPDH-MAPK6-LBD1 module controls drought-responsive transcription",
  "title_text": "A phosphorylation-gated GAPDH-MAPK6-LBD1 module controls drought-responsive transcription",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:53Z",
  "url": "https://research.draconitic.com/paper/7q9f2g11pcep1",
  "venue": "Science Advances",
  "versions": []
}