{
  "abstract": "Recombineering enables precise genome engineering across diverse bacteria, yet efficient tools for large DNA integration remain underdeveloped for Streptomyces and other biotechnologically relevant bacteria. Here we identify a panel of highly active RecET recombinase pairs from actinobacteriophages that mediate efficient homologous recombination in Streptomyces. The lead candidate, Che9cRecET, supports genomic integration of kilobase-scale DNA with only 50bp homology arms and enables accurate insertion of 79kb biosynthetic gene clusters, far outperforming conventional genome editing approaches. Structural and biochemical analyses reveal Che9cRecE as a distinct C3-symmetric toroidal homotrimeric 5'-3' exonuclease, whose extended C-terminal loop acts as a tunable rheostat calibrating exonuclease activity to an optimal window for recombination. We further show this portable C-terminal loop enhances recombineering efficiency across phylogenetically diverse exonucleases including Escherichia coli Redalpha/RecE and Pseudomonas A. Our work uncovers a conserved regulatory mechanism of the RecE/Redalpha exonuclease family and delivers a generalizable strategy for advancing bacterial genome editing.",
  "abstract_shown": true,
  "affiliations": [
    "Shandong University"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "name": "J. Luan"
    },
    {
      "name": "C. Jiang"
    },
    {
      "name": "R. Guo"
    },
    {
      "affs": [
        0
      ],
      "name": "C. Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "S. Wang"
    },
    {
      "name": "A. F. Stewart"
    },
    {
      "name": "J. Fu"
    },
    {
      "name": "Y. Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "H. Wang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7qsv18bwn7bnr.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.29.762211",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.29.762211v1.full",
  "id": "7qsv18bwn7bnr",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.29.762211v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "molecular biology"
    ]
  ],
  "openalex": null,
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.29.762211v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-29",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/7qsv18bwn7bnr.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:30:37Z",
      "native_id": "10.64898/2026.10.29.762211",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.29.762211v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "biorxiv:molecular biology",
      "subfield": "molecular-biology"
    }
  ],
  "title": "A portable C-terminal rheostat calibrates RecE/Redα exonuclease activity for enhanced bacterial recombineering",
  "title_text": "A portable C-terminal rheostat calibrates RecE/Redα exonuclease activity for enhanced bacterial recombineering",
  "type": "preprint",
  "updated_at": "2026-11-01T00:20:45Z",
  "url": "https://research.draconitic.com/paper/7qsv18bwn7bnr",
  "venue": "bioRxiv",
  "versions": []
}