{
  "abstract": "Immobilized antigen induces an immune synapse, which is supported by Swiprosin-1/EFhd2 (EFhd2) in B and T cells. Here, we find that EFhd2 regulates mitochondrial fitness in the B cell synapse. Whereas activated EFhd2-deficient (KO) B cells show higher mitochondrial mass than wildtype (WT) B cells, this potential advantage reverses on coated anti-IgM antibodies: activated WT B cells enhance basal and maximal respiration as well as proton leak, with large increments of mitochondrial spare capacity, but EFhd2KO B cells fail to do so. Actin destabilization, but much more so microtubule destabilization, impairs mitochondrial up-regulation in WT, but not in EFhd2KO B cells. Conversely, microtubule stabilization, and to a lesser extent actin stabilization, restore mitochondrial spare capacity in EFhd2KO B cells at the BCR synapse. EFhd2KO B cells fail to coordinate the microtubule organization center, mitochondria and IgM BCRs upon synapse formation. Hence, microtubule-dependent mitochondrial organization at the immune synapse coordinates mitochondrial fitness in B cells via EFhd2.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Translational Immunology, Department of Medicine 3 - Rheumatology and Immunology, Nikolaus-Fiebiger Center for Molecular Medicine, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Uniklinikum Erlangen",
    "Deutsches Zentrum Immuntherapie, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Uniklinikum Erlangen",
    "Optical Imaging Competence Centre Erlangen (FAU OICE), Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg",
    "FAU Profile Center Immunomedicine, FAU Erlangen-Nürnberg",
    "Department of Medicine 3 - Rheumatology and Immunology, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Uniklinikum Erlangen"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Leonie Weckwerth",
      "orcid": "0009-0005-2959-3975"
    },
    {
      "affs": [
        2
      ],
      "name": "Benjamin Schmid",
      "orcid": "0000-0002-9327-2296"
    },
    {
      "affs": [
        2
      ],
      "name": "Philipp Tripal",
      "orcid": "0000-0003-0568-1725"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Ann-Kathrin Himmelreich"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Jana Thomas"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Katharina Pracht",
      "orcid": "0000-0002-8943-4897"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Sophia Urbanczyk"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Hannah Allnach"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Anna Gottwald"
    },
    {
      "affs": [
        1,
        3,
        4
      ],
      "name": "Georg Schett"
    },
    {
      "affs": [
        2
      ],
      "name": "Ralph Palmisano",
      "orcid": "0000-0003-4283-2115"
    },
    {
      "affs": [
        0,
        1,
        3
      ],
      "name": "Dirk Mielenz",
      "orcid": "0000-0002-1135-0642"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7t02zd8fzv840.bib",
  "cited_by": 0,
  "doi": "10.26508/lsa.202603876",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13736178/",
  "id": "7t02zd8fzv840",
  "issns": [
    "2575-1077"
  ],
  "landing_url": "https://www.life-science-alliance.org/lookup/doi/10.26508/lsa.202603876",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "12"
    ],
    [
      "heading",
      "14"
    ],
    [
      "heading",
      "4"
    ],
    [
      "heading",
      "Research Article"
    ],
    [
      "heading",
      "Research Articles"
    ]
  ],
  "openalex": null,
  "pdf_url": null,
  "pmcid": "PMC13736178",
  "pmid": "42982553",
  "published": "2026-11-04",
  "publisher": "Life Science Alliance, LLC",
  "ris": "https://research.draconitic.com/paper/7t02zd8fzv840.ris",
  "sources": [
    {
      "first_seen": "2026-11-05T00:21:04Z",
      "native_id": "10.26508/lsa.202603876",
      "source": "crossref",
      "url": "https://www.life-science-alliance.org/lookup/doi/10.26508/lsa.202603876"
    },
    {
      "first_seen": "2026-11-09T12:21:41Z",
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      "source": "pmc",
      "url": "https://doi.org/10.26508/lsa.202603876"
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  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "journal-openalex:1312|Genomics and Chromatin Dynamics",
      "subfield": "molecular-biology"
    },
    {
      "field": "biology",
      "origin": "issn:2575-1077",
      "subfield": "general"
    }
  ],
  "title": "EFhd2 regulates microtubule-dependent mitochondrial fitness at the B cell immune synapse",
  "title_text": "EFhd2 regulates microtubule-dependent mitochondrial fitness at the B cell immune synapse",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T12:21:41Z",
  "url": "https://research.draconitic.com/paper/7t02zd8fzv840",
  "venue": "Life Science Alliance",
  "versions": []
}