{
  "abstract": "Angiogenesis is a key process in colorectal cancer (CRC) progression, with vascular endothelial growth factor A (VEGFA) being a major pro-angiogenic mediator. miR-195-5p has previously been identified by our group as a tumor-suppressive miRNA downregulated in CRC. Here, we aimed to investigate whether miR-195-5p contributes to CRC-associated angiogenenic processes through VEGFA regulation. Potential miR-195-5p targets were investigated through bioinformatic predictions and miRNA Target RNA immunoprecipitation. VEGFA expression was evaluated in public CRC datasets and paired tumor/adjacent tissues of a cohort of CRC patients by quantitative real-time PCR and immunohistochemistry. miR-195-5p gain- and loss-of-function experiments were performed in CRC cells to assess VEGFA and VEGFR2 expression and extracellular VEGF-A levels by ELISA. Endothelial tube formation assays using HUVECs were conducted to evaluate paracrine angiogenic effects. VEGFA expression was also analyzed in an AOM/DSS-induced murine CRC model treated with a miR-195-5p mimic. VEGFA was predicted as a conserved miR-195-5p target and was enriched in miR-195-5p-associated RISCs. VEGFA was overexpressed in CRC tissues compared to normal or adjacent non-tumor tissues. miR-195-5p restoration reduced VEGFA mRNA and protein expression, extracellular VEGF-A levels, and VEGFR2 protein levels in the HCT116 CRC cell line, whereas miR-195-5p inhibition produced the opposite molecular profile. Conditioned media from miR-195-5p-overexpressing CRC cells significantly impaired endothelial tube formation. Consistently, miR-195-5p in vivo administration reduced colonic Vegfa expression in AOM/DSS-treated mice. Overall, these findings indicate that miR-195-5p negatively modulates VEGFA expression and reduces the ability of CRC cell-derived conditioned media to support endothelial tube formation. This study extends the tumor-suppressive functions previously attributed to miR-195-5p by supporting its involvement in the regulation of angiogenesis-related processes in CRC.",
  "abstract_shown": true,
  "affiliations": [
    "National Institute of Gastroenterology S. De Bellis, IRCCS Research Hospital, Via Turi 27, 70013 Castellana Grotte, BA, Italy"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Emanuele Piccinno",
      "orcid": "0000-0002-7988-1917"
    },
    {
      "affs": [
        0
      ],
      "name": "Viviana Scalavino",
      "orcid": "0000-0002-0273-2825"
    },
    {
      "affs": [
        0
      ],
      "name": "Giusy Bianco"
    },
    {
      "affs": [
        0
      ],
      "name": "Serena Mancarella",
      "orcid": "0000-0003-2692-1169"
    },
    {
      "affs": [
        0
      ],
      "name": "Ilaria Grassi",
      "orcid": "0009-0001-7940-4977"
    },
    {
      "affs": [
        0
      ],
      "name": "Maria Teresa Savino",
      "orcid": "0009-0004-5822-1268"
    },
    {
      "affs": [
        0
      ],
      "name": "Raffaele Armentano",
      "orcid": "0000-0003-1661-5504"
    },
    {
      "affs": [
        0
      ],
      "name": "Gianluigi Giannelli",
      "orcid": "0000-0002-5140-8060"
    },
    {
      "affs": [
        0
      ],
      "name": "Grazia Serino",
      "orcid": "0000-0002-2971-0802"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7t5330xgnz1er.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27219971",
  "fulltext_url": null,
  "id": "7t5330xgnz1er",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/21/9971",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-06",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/7t5330xgnz1er.ris",
  "sources": [
    {
      "first_seen": "2026-11-10T00:21:27Z",
      "native_id": "10.3390/ijms27219971",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/21/9971"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "issn:1422-0067",
      "subfield": "general"
    }
  ],
  "title": "miR-195-5p Reduces VEGFA Expression and Angiogenesis in Colorectal Cancer",
  "title_text": "miR-195-5p Reduces VEGFA Expression and Angiogenesis in Colorectal Cancer",
  "type": "peer-reviewed",
  "updated_at": "2026-11-10T00:21:27Z",
  "url": "https://research.draconitic.com/paper/7t5330xgnz1er",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}