{
  "abstract": "Background:: Polycystic ovary syndrome (PCOS) is a prevalent metabolic and reproductive disorder and one of the leading causes of infertility. Bone marrow-derived mesenchymal stem cells (BM-MSCs) possess anti-inflammatory properties and have shown potential in alleviating PCOS manifestations in experimental models. This study aimed to evaluate the therapeutic efficacy of transplanted BM-MSCs and BM-MSC-derived culture media in a letrozole (LTZ)-induced PCOS rat model.\n\nMaterials and Methods:: The experimental study was conducted on 50 female Sprague Dawley rats aged (4-6 weeks) and weighing (100-120 g), using a LTZ-induced PCOS model. Animals were randomly assigned to 4 groups, including control, PCOS, BM-MSC-treated, and BM-MSC culture media-treated groups. BM-MSCs were isolated from (bone marrow) and administered via (tail vain) at a dose of (1×106 BM-MSC). Blood samples were collected to measure hormonal and metabolic parameters (insulin, androgen, progesterone, estrogen, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) using [enzyme-linked immunosorbent assay (ELISA) technique). Ovarian tissues were analyzed by for histopathological and immunohistochemical methods to assess [key markers, e.g., follicular development, tumor necrosis factor-alpha (TNF), and vascular endothelial growth factor (VEGF)].\n\nResults:: The PCOS group exhibited hormonal imbalance, metabolic disturbance, and ovarian dysfunction. BM-MSC treatment significantly reduced insulin (P=0.003) and androgen levels (P=0.040), while restoring progesterone (P=0.003), estrogen (P=0.007), FSH (P=0.009), and LH (P=0.001) to near-normal levels. BM-MSCs markedly improved both metabolic and reproductive parameters, effectively restoring fertility in the PCOS model. Treatment with BM-MSC-derived culture media produced partial improvement but did not fully reverse PCOS manifestations.\n\nConclusion:: BM-MSCs improved ovarian structure and function, ameliorating both metabolic and reproductive dysfunction in the PCOS rat model. BM-MSC transplantation yielded the most favorable outcomes, whereas BM-MSC-derived culture media alone provided incomplete therapeutic benefits.",
  "abstract_shown": true,
  "affiliations": [
    "Zoology Department, Faculty of Science, Mansoura University, Mansoura, Egypt",
    "Medical Experimental Research Center (MERC), Faculty of Medicine, Mansoura University, Mansoura, Egypt",
    "Department of Biotechnology, College of Science, Taif University, Taif, Saudi Arabia",
    "Zoology Department, Urology and Nephrology Center, Mansoura University, Mansoura, Egypt",
    "Oral Biology Department, Faculty of Oral and Dental Medicine, Delta University for Science and Technology, Gamassa, Egypt"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Amoura M. Abou-El-Naga"
    },
    {
      "affs": [
        0
      ],
      "name": "Nada Younes Elarby"
    },
    {
      "affs": [
        1
      ],
      "name": "Sara El-dusoky"
    },
    {
      "affs": [
        2
      ],
      "name": "Eman Fayad"
    },
    {
      "affs": [
        3
      ],
      "name": "Mohamed Ahmed Sobh"
    },
    {
      "affs": [
        4
      ],
      "name": "Reham A. Ghanem"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7v8f1qpwf2zqm.bib",
  "cited_by": 0,
  "doi": "10.22074/ijfs.2025.2015395.1568",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13710901/",
  "id": "7v8f1qpwf2zqm",
  "issns": [
    "2008-076X",
    "2008-0778"
  ],
  "landing_url": "https://doi.org/10.22074/ijfs.2025.2015395.1568",
  "language": "en",
  "licence": "CC-BY-NC-3.0",
  "licence_label": "CC BY-NC 3.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/3.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Gynecology and Female Infertility"
    ],
    [
      "heading",
      "Original Article"
    ],
    [
      "heading",
      "Stem Cells Biology"
    ],
    [
      "openalex",
      "2743|Ovarian function and disorders"
    ]
  ],
  "openalex": "W7223357749",
  "pdf_url": null,
  "pmcid": "PMC13710901",
  "pmid": "42929028",
  "published": "2026-10-03",
  "publisher": "Royan Institute",
  "ris": "https://research.draconitic.com/paper/7v8f1qpwf2zqm.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T12:21:46Z",
      "native_id": "PMC13710901",
      "source": "pmc",
      "url": "https://doi.org/10.22074/ijfs.2025.2015395.1568"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2743|Ovarian function and disorders",
      "subfield": "reproductive-child"
    }
  ],
  "title": "Therapeutic Potential of Bone Marrow-Derived Mesenchymal Stem Cells in A Letrozole-Induced Rat Model of Polycystic Ovary Syndrome",
  "title_text": "Therapeutic Potential of Bone Marrow-Derived Mesenchymal Stem Cells in A Letrozole-Induced Rat Model of Polycystic Ovary Syndrome",
  "type": "peer-reviewed",
  "updated_at": "2026-10-31T12:21:46Z",
  "url": "https://research.draconitic.com/paper/7v8f1qpwf2zqm",
  "venue": "International Journal of Fertility & Sterility",
  "versions": []
}