{
  "abstract": "Natural polysaccharides possess diverse biological functions, yet their macromolecular architecture frequently restricts molecular accessibility and limits their application as health-promoting ingredients. Here, we employed an ultrasound-driven strategy to remodel the molecular structure of raspberry polysaccharide (RAP) and investigated how controlled depolymerization governs its intestinal barrier-protective properties. Native RAP was subjected to ultrasonic irradiation for 2 and 5 h, generating two structurally tailored derivatives, RAP-2 and RAP-5. Ultrasonic processing progressively decreased the molecular size of RAP from 10.47 kDa to 8.79 and 5.94 kDa, respectively, without disrupting its monosaccharide profile or fundamental chemical framework. Structural analyses revealed that ultrasound-induced cavitation promoted polysaccharide chain scission, leading to a transition from compact aggregates toward fragmented and porous architectures. Importantly, molecular remodeling translated into enhanced biological functions. In an LPS-challenged Caco-2/THP-1 macrophage co-culture model, RAP-5 exhibited superior capacity to preserve epithelial barrier integrity, restoring TEER values to 93.28% while markedly attenuating inflammatory mediator release, including IL-6 (65.49 pg/mL), TNF-α (271.63 pg/mL), and IL-1β (94.91 pg/mL). Moreover, RAP-5 effectively recovered the expression of barrier-associated genes, increasing ZO-1 and Occludin transcripts to 0.89- and 0.82-fold of the control levels, respectively. Collectively, these findings demonstrate that controlled ultrasonic remodeling represents an efficient approach for optimizing the structure-function relationship of RAP, providing new opportunities for developing polysaccharide-based functional foods targeting intestinal homeostasis.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Neurosurgery, Huzhou Central Hospital, Fifth School of Clinical Medicine of Zhejiang Chinese Medical University, Huzhou, Zhejiang 313000, China; Enshi Tujia and Miao Autonomous Prefecture Academy of Agricultural Sciences, Enshi, Hubei 445000, China.",
    "Guangdong Provincial Key Laboratory of Intelligent Port Security Inspection, Huangpu Customs District P.R. China, Guangzhou, Guangdong 510700, China; Huangpu Customs District Technology Center, Dongguan, Guangdong 523000, China.",
    "Enshi Tujia and Miao Autonomous Prefecture Academy of Agricultural Sciences, Enshi, Hubei 445000, China.",
    "School of Food Science and Engineering, South China University of Technology, Guangzhou, Guangdong 510640, China.",
    "School of Health Science and Engineering, Shanghai Engineering Research Center of Food Rapid Detection, University of Shanghai for Science and Technology, Shanghai 200093, China.",
    "Enshi Tujia and Miao Autonomous Prefecture Academy of Agricultural Sciences, Enshi, Hubei 445000, China; School of Food Science and Engineering, South China University of Technology, Guangzhou, Guangdong 510640, China; College of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021, China. Electronic address: dzman320@163.com.",
    "Department of Neurosurgery, Huzhou Central Hospital, Fifth School of Clinical Medicine of Zhejiang Chinese Medical University, Huzhou, Zhejiang 313000, China. Electronic address: jiangsu@i.smu.edu.cn."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Qiong Deng"
    },
    {
      "affs": [
        1
      ],
      "name": "Xuelin Huang"
    },
    {
      "affs": [
        1
      ],
      "name": "Peihua Liu"
    },
    {
      "affs": [
        2
      ],
      "name": "Baishun Hu"
    },
    {
      "affs": [
        3
      ],
      "name": "Bin Zhang"
    },
    {
      "affs": [
        4
      ],
      "name": "Fengqin Yin"
    },
    {
      "affs": [
        5
      ],
      "name": "Zuman Dou"
    },
    {
      "affs": [
        6
      ],
      "name": "Feng Zhang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7vgegtxrtn89j.bib",
  "cited_by": 0,
  "doi": "10.1016/j.ultsonch.2026.108109",
  "fulltext_url": "https://europepmc.org/articles/PMC13679305",
  "id": "7vgegtxrtn89j",
  "issns": [
    "1350-4177",
    "1873-2828"
  ],
  "landing_url": "https://europepmc.org/article/MED/42863375",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1106|Polysaccharides Composition and Applications"
    ]
  ],
  "openalex": "W7221458248",
  "pdf_url": "https://europepmc.org/articles/PMC13679305?pdf=render",
  "pmcid": "PMC13679305",
  "pmid": "42863375",
  "published": "2026-10-11",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/7vgegtxrtn89j.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:00Z",
      "native_id": "MED:42863375",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42863375"
    }
  ],
  "subjects": [],
  "title": "Ultrasonic modification on the physicochemical properties, structure characterizations, and intestinal barrier-protective activities of raspberry polysaccharides.",
  "title_text": "Ultrasonic modification on the physicochemical properties, structure characterizations, and intestinal barrier-protective activities of raspberry polysaccharides.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:00Z",
  "url": "https://research.draconitic.com/paper/7vgegtxrtn89j",
  "venue": "Ultrasonics sonochemistry",
  "versions": []
}