{
  "abstract": "Background\n\nSepsis-associated encephalopathy (SAE) is a common and severe complication in septic patients, characterized by the increased mortality and cognition dysfunction. N6-Methyladenosine (m⁶A), one of the most prevalent RNA modifications, plays crucial roles in the central nervous system by regulating RNA metabolism. However, its involvement in the pathogenesis of SAE remains unexplored.Methods\n\nRNA sequencing (RNA-seq) and methylated RNA immunoprecipitation (MeRIP)-sequencing were performed to analyze the transcriptomic profiles and m⁶A methylation in the brain cortex of LPS-induced septic mice. Bioinformatics analysis was carried out to identify genes with altered m⁶A methylation and differential expression. Target genes and m⁶A regulators were further validated using quantitative reverse transcription polymerase chain reaction (RT-qPCR).Results\n\nNumerous genes exhibited differential expression and m⁶A modification in the cortical tissues of septic mice. Gene function enrichment analysis revealed that genes with differential expression were enriched in pathways related to immune and inflammatory responses as well as neuroactive ligand-receptor interaction, and genes with differential m⁶A modification showed significant associations with pathways related to neurodegeneration, synapse function and metabolism. Comprehensive analysis of genes with both altered m⁶A methylation and transcription identified several key genes highly associated with inflammatory processes. Validation experiments confirmed significant dysregulation of mRNA levels for several target genes (Ahsg, Alb, Apoa1, Serpina1b, Serpinf2, and Serpina3K) and m⁶A regulators (Mettl3, Mettl16, Igfbp1 and Igfbp3) in the cortex of septic mice.Conclusion\n\nThis study provided a transcriptome-wide analysis of m⁶A methylation in the cortical tissues of septic mice, suggesting that m⁶A-mediated regulation of inflammatory gene expression is associated with the pathogenesis of SAE. The findings presented valuable insights into the mechanisms of sepsis-associated brain injury and potential therapeutic targets.",
  "abstract_shown": true,
  "affiliations": [
    "Maternal and Child Healthcare Medical Research Institute, Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, Shenzhen, People's Republic of China.",
    "Hengyang Medical School, University of South China, Hengyang, People's Republic of China.",
    "Department of Critical Care Medicine, Shenzhen Third People's Hospital, Shenzhen, People's Republic of China.",
    "Center for Medical Genetics, School of Life Sciences, Central South University, Changsha, People's Republic of China.",
    "Department of Anesthesiology, Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, Shenzhen, People's Republic of China.",
    "Shenzhen Key Laboratory of Maternal and Child Health and Diseases, Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, Shenzhen, People's Republic of China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Juan Zhao"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Tengfei Zhu"
    },
    {
      "affs": [
        1
      ],
      "name": "Chao Zhao"
    },
    {
      "affs": [
        3
      ],
      "name": "Xu Huang"
    },
    {
      "affs": [
        0
      ],
      "name": "Fang Yao"
    },
    {
      "affs": [
        0
      ],
      "name": "Yueyuan Qin"
    },
    {
      "affs": [
        0
      ],
      "name": "Baojiang Wang"
    },
    {
      "affs": [
        4
      ],
      "name": "Gehui Li"
    },
    {
      "affs": [
        0,
        5
      ],
      "name": "Shan Duan"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7vpm0y16z4twa.bib",
  "cited_by": 0,
  "doi": "10.2147/jir.s586027",
  "fulltext_url": "https://europepmc.org/articles/PMC13701088",
  "id": "7vpm0y16z4twa",
  "issns": [
    "1178-7031"
  ],
  "landing_url": "https://europepmc.org/article/MED/42936262",
  "language": "eng",
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|RNA modifications and cancer"
    ]
  ],
  "openalex": "W7223169645",
  "pdf_url": "https://europepmc.org/articles/PMC13701088?pdf=render",
  "pmcid": "PMC13701088",
  "pmid": "42936262",
  "published": "2026-10-22",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/7vpm0y16z4twa.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:12Z",
      "native_id": "MED:42936262",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42936262"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|RNA modifications and cancer",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Integrative Analysis of m⁶A Methylation and Transcriptomic Profiles in Cortical Tissues of Mice with Sepsis-Associated Encephalopathy.",
  "title_text": "Integrative Analysis of m⁶A Methylation and Transcriptomic Profiles in Cortical Tissues of Mice with Sepsis-Associated Encephalopathy.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:22:12Z",
  "url": "https://research.draconitic.com/paper/7vpm0y16z4twa",
  "venue": "Journal of inflammation research",
  "versions": []
}