{
  "abstract": "BACKGROUND: Methicillin-resistant Staphylococcus aureus (MRSA) is a leading human pathogen, and declining antibiotic efficacy has renewed interest in lytic bacteriophages, whose efficacy phenotypic assays predict poorly.\n\nOBJECTIVES: To characterise the lysis and host-recognition modules of lytic S. aureus bacteriophages, and to determine how far the detectability of each module depends on the annotation source rather than on the genomes.\n\nMETHODS: Twenty-two S. aureus phage genomes from NCBI GenBank were processed through an identical detection funnel under three annotation sources: the deposited record, Pharokka and Phold. Modules were validated by InterPro, disrupted endolysins resolved by tBLASTn, and each domain aligned separately to measure identity.\n\nFINDINGS: The LysK-type endolysin (CHAP-Amidase-SH3b) was conserved and detected identically under all three sources. Tail fibre/receptor-binding protein (RBP) detection was strongly source-dependent: 14 of 22 genomes from the deposited annotation, 20 of 22 after re-annotation, and 22 of 22 combined. Resolved by domain, conservation was scale-dependent: the amidase domain was invariant within species while SH3b varied by 5.7%.\n\nMAIN CONCLUSIONS: Lysis-module conservation is independent of the annotation source; the apparent variability of host recognition is largely an artefact of incomplete deposited annotation. Module-level surveys require re-annotation before comparative claims.",
  "abstract_shown": true,
  "affiliations": [
    "Universitas Terbuka, Faculty of Science and Technology, Department of Biology, Tangerang Selatan, Indonesia"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 2,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Ivan Armadi Hasugian",
      "orcid": "0009-0006-7501-891X"
    },
    {
      "affs": [
        0
      ],
      "name": "Na’ilah Insani Alifiyah"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7vvzbyk209fdv.bib",
  "cited_by": 0,
  "doi": "10.1590/0074-02760260245",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13729910/?tool=EBI",
  "id": "7vvzbyk209fdv",
  "issns": [
    "0074-0276",
    "1678-8060"
  ],
  "landing_url": "https://europepmc.org/article/PMC/PMC13729910",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research Article"
    ],
    [
      "openalex",
      "2406|Bacteriophages and microbial interactions"
    ]
  ],
  "openalex": "W7165020432",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13729910/pdf/?tool=EBI",
  "pmcid": "PMC13729910",
  "pmid": "42987105",
  "published": "2026-11-02",
  "publisher": "Instituto Oswaldo Cruz",
  "ris": "https://research.draconitic.com/paper/7vvzbyk209fdv.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T15:20:46Z",
      "native_id": "PMC:PMC13729910",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13729910"
    },
    {
      "first_seen": "2026-11-07T12:22:38Z",
      "native_id": "PMC13729910",
      "source": "pmc",
      "url": "https://doi.org/10.1590/0074-02760260245"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2406|Bacteriophages and microbial interactions",
      "subfield": "microbiology"
    }
  ],
  "title": "In silico characterisation of lysis and host-recognition modules in Staphylococcus aureus bacteriophage genomes",
  "title_text": "In silico characterisation of lysis and host-recognition modules in Staphylococcus aureus bacteriophage genomes",
  "type": "peer-reviewed",
  "updated_at": "2026-11-07T12:22:38Z",
  "url": "https://research.draconitic.com/paper/7vvzbyk209fdv",
  "venue": "Memorias do Instituto Oswaldo Cruz",
  "versions": []
}