{
  "abstract": "This study aimed to develop a one-step loop-mediated isothermal amplification combined with a lateral flow dipstick (LAMP-LFD) DNA biosensor for the detection of α0-thalassemia (SEA deletion). Genomic DNA was obtained from 48 α0-thalassemia (SEA deletion)-positive and 48 (SEA deletion)-negative (normal) Thai subjects. DNA was analyzed by LAMP assay at 61°C for 60 min using specific primers for the α0-thalassemia (SEA deletion) type. Following amplification, the products were visually detected using an LFD, with results available within 5–10 min. The one-step LAMP-LFD DNA biosensor could detect DNA carrying the α0-thalassemia (SEA deletion) type at levels as low as 200 pg, with 100% sensitivity and 100% specificity. Furthermore, no cross-reactivity was observed with DNA carrying other α0-thalassemia deletion types (THAI deletion). The LAMP-LFD DNA biosensor is useful for identifying carriers of α0-thalassemia (SEA deletion) (the most common deletion type found in Thailand and across Southeast Asia). This novel one-step LAMP-LFD kit will be beneficial for medical laboratory technologists performing genetic carrier screening tests as it is simple to perform and requires minimal equipment and time. Furthermore, this method supports clinicians in providing genetic counseling and implementing preventive strategies against severe thalassemia, a significant public health concern in Thailand.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Clinical Pathology, Faculty of Medicine Vajira Hospital, Navamindradhiraj University, Bangkok, Thailand",
    "Department of Pathology, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand",
    "Department of Pediatrics, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand",
    "Innovative Learning Center, Srinakharinwirot University, Thailand"
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Jutturong Ckumdee"
    },
    {
      "affs": [
        1
      ],
      "name": "Sataron Karnjanaopas"
    },
    {
      "affs": [
        2
      ],
      "name": "Duantida Songdej"
    },
    {
      "affs": [
        3
      ],
      "name": "Noppawan Tungbuppha"
    },
    {
      "name": "Thongchai Kaewphinit"
    },
    {
      "name": "Thanwa Wongsuk"
    },
    {
      "name": "Chanita Sanyear"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7wganj992gctx.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pone.0360555",
  "fulltext_url": null,
  "id": "7wganj992gctx",
  "issns": [
    "1932-6203"
  ],
  "landing_url": "https://doi.org/10.1371/journal.pone.0360555",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2204|Biosensors and Analytical Detection"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Genomics"
    ],
    [
      "plos",
      "/Biology and life sciences/Molecular biology/Molecular biology techniques/Artificial gene amplification and extension/Polymerase chain reaction"
    ],
    [
      "plos",
      "/Biology and life sciences/Molecular biology/Molecular biology techniques/Molecular probe techniques/DNA probes"
    ],
    [
      "plos",
      "/Engineering and technology/Equipment/Detectors/Biosensors"
    ],
    [
      "plos",
      "/Medicine and health sciences/Clinical genetics/Genetic diseases/Autosomal recessive diseases/Thalassemia"
    ],
    [
      "plos",
      "/Medicine and health sciences/Clinical genetics/Genetic diseases/Autosomal recessive diseases/Thalassemia/Alpha-thalassemia"
    ],
    [
      "plos",
      "/Medicine and health sciences/Hematology/Hemoglobinopathies/Thalassemia"
    ],
    [
      "plos",
      "/Medicine and health sciences/Hematology/Hemoglobinopathies/Thalassemia/Alpha-thalassemia"
    ],
    [
      "plos",
      "/Medicine and health sciences/Medical conditions/Genetic diseases/Autosomal recessive diseases/Thalassemia"
    ],
    [
      "plos",
      "/Medicine and health sciences/Medical conditions/Genetic diseases/Autosomal recessive diseases/Thalassemia/Alpha-thalassemia"
    ],
    [
      "plos",
      "/Research and analysis methods/Bioassays and physiological analysis/Biochemical analysis/Colorimetric assays"
    ],
    [
      "plos",
      "/Research and analysis methods/Electrophoretic techniques/Gel electrophoresis/Agarose gel electrophoresis"
    ],
    [
      "plos",
      "/Research and analysis methods/Molecular biology techniques/Artificial gene amplification and extension/Polymerase chain reaction"
    ],
    [
      "plos",
      "/Research and analysis methods/Molecular biology techniques/Molecular probe techniques/DNA probes"
    ]
  ],
  "openalex": "W7221513657",
  "pdf_url": "https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0360555&type=printable",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-12",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/7wganj992gctx.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:30Z",
      "native_id": "10.1371/journal.pone.0360555",
      "source": "plos",
      "url": "https://doi.org/10.1371/journal.pone.0360555"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "plos:molecular biology",
      "subfield": "molecular-biology"
    },
    {
      "field": "biology",
      "origin": "plos:genomics",
      "subfield": "genetics-genomics"
    }
  ],
  "title": "Development of loop-mediated isothermal amplification (LAMP) combined with a lateral flow dipstick DNA biosensor for detection of α thalassemia 1 [Southeast Asia (SEA), --SEA deletion]",
  "title_text": "Development of loop-mediated isothermal amplification (LAMP) combined with a lateral flow dipstick DNA biosensor for detection of α thalassemia 1 [Southeast Asia (SEA), --SEA deletion]",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:30Z",
  "url": "https://research.draconitic.com/paper/7wganj992gctx",
  "venue": "PLOS One",
  "versions": []
}