{
  "abstract": "Although the original Hermes transposon mutagenesis system has proven successful for saturated insertional mutagenesis in Candida glabrata, its reliance on uracil auxotrophy may limit its applicability under nutrient-restricted host environments. To overcome this limitation, Zaeske et al. (Microbiol Spectr 14:e0084826, 2026, https://doi.org/10.1128/spectrum.00848-26) recently developed a prototrophic alternative that uses cycloheximide (CHX) instead of 5-fluoroorotic acid (5-FOA) to enrich for transposon insertion mutants, generating high-complexity pools. This system provides a valuable resource for investigating the genetic and cellular determinants of colonization and pathogenicity in this important fungal pathogen.",
  "abstract_shown": true,
  "affiliations": [
    "State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China"
  ],
  "article_type": "article commentary",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Junhua Wan",
      "orcid": "0000-0002-5762-9956"
    },
    {
      "affs": [
        0
      ],
      "name": "Yuru Guo",
      "orcid": "0009-0001-5432-9130"
    },
    {
      "affs": [
        0
      ],
      "name": "Yabing Song"
    },
    {
      "affs": [
        0
      ],
      "name": "Jiaxin Gao",
      "orcid": "0000-0001-6161-4629"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7xsfmpxp8byp6.bib",
  "cited_by": 0,
  "doi": "10.1128/spectrum.02529-26",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13729112/",
  "id": "7xsfmpxp8byp6",
  "issns": [
    "2165-0497"
  ],
  "landing_url": "https://doi.org/10.1128/spectrum.02529-26",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Commentary"
    ],
    [
      "openalex",
      "1312|Fungal and yeast genetics research"
    ]
  ],
  "openalex": "W7214609501",
  "pdf_url": null,
  "pmcid": "PMC13729112",
  "pmid": "42803559",
  "published": "2026-09-28",
  "publisher": "American Society for Microbiology (ASM)",
  "ris": "https://research.draconitic.com/paper/7xsfmpxp8byp6.ris",
  "sources": [
    {
      "first_seen": "2026-11-07T12:21:56Z",
      "native_id": "PMC13729112",
      "source": "pmc",
      "url": "https://doi.org/10.1128/spectrum.02529-26"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Fungal and yeast genetics research",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Version 2.0 of the Hermes transposon-based mutagenesis system for prototrophic mutant pool generation in Candida glabrata",
  "title_text": "Version 2.0 of the Hermes transposon-based mutagenesis system for prototrophic mutant pool generation in Candida glabrata",
  "type": "peer-reviewed",
  "updated_at": "2026-11-07T12:21:56Z",
  "url": "https://research.draconitic.com/paper/7xsfmpxp8byp6",
  "venue": "Microbiology Spectrum",
  "versions": []
}