{
  "abstract": "Plant cell cultures underpin regeneration, transformation and emerging applications in biotechnology and plant cell agriculture, yet the genetic determinants of proliferative performance remain poorly characterized. Here, we combined large-scale forward genetics with automated image-based phenotyping to quantify 27,648 calli representing 2,880 Arabidopsis thaliana T-DNA insertion lines. Of these, 2,456 lines produced calli smaller than the WT population mean, whereas only 190 produced larger calli, revealing a strongly asymmetric phenotypic distribution. Enhanced callus growth was not consistently associated with increased vegetative growth, indicating that these traits can be genetically uncoupled. Validation of selected high-growth mutants in suspension culture identified lines with enhanced biomass accumulation, while serial propagation revealed independent variation in sustained proliferative capacity. Thus, callus growth, whole-plant growth, short-term suspension biomass accumulation and sustained proliferation represent distinct, not necessarily coupled components of proliferative performance. These findings establish a scalable framework for identifying genetic determinants relevant to the development of improved plant cell production lines.",
  "abstract_shown": true,
  "affiliations": [
    "Universidad Politecnica de Cartagena, member of European University of Technology EUT+"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "name": "F. Verdu-Navarro"
    },
    {
      "name": "A. Espin-Veracruz"
    },
    {
      "name": "J. Weiss"
    },
    {
      "name": "J. Kacprzyk"
    },
    {
      "name": "P. J. Navarro"
    },
    {
      "name": "D. Parras"
    },
    {
      "name": "D. Borraz-Moron"
    },
    {
      "name": "E. Salas"
    },
    {
      "affs": [
        0
      ],
      "name": "M. Egea-Cortines"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7z4b21dz52f4v.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.30.762808",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.30.762808v1.full",
  "id": "7z4b21dz52f4v",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.30.762808v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "plant biology"
    ]
  ],
  "openalex": null,
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.30.762808v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-30",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/7z4b21dz52f4v.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T00:21:30Z",
      "native_id": "10.64898/2026.10.30.762808",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.30.762808v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:plant biology",
      "subfield": "plant-biology"
    }
  ],
  "title": "Large-scale forward genetics reveals genetically separable components of plant cell proliferative capacity",
  "title_text": "Large-scale forward genetics reveals genetically separable components of plant cell proliferative capacity",
  "type": "preprint",
  "updated_at": "2026-11-05T00:20:52Z",
  "url": "https://research.draconitic.com/paper/7z4b21dz52f4v",
  "venue": "bioRxiv",
  "versions": []
}