{
  "abstract": "Rapid and reliable methods are required for environmental monitoring of unintended releases of genetically modified (GM) crops. We developed a real-time recombinase polymerase amplification (RPA) assay targeting the bar gene for the rapid detection of GM soybean and rice, combined with a simplified NaOH-based DNA extraction method for field applications. Primer-probe set 4 provided the most specific and reliable detection, while soybean lectin and rice actin served as internal positive controls. Lectin and actin were consistently detected in GM and non-GM samples, respectively. A 0.1 N NaOH extraction method produced DNA of sufficient quality for direct real-time RPA without additional purification. The integrated assay specifically detected the bar gene in GM samples, with no amplification observed in non-GM samples, and generated results within approximately 25 min at 39 °C. This assay provides a rapid, simple, and field-deployable approach for the detection and environmental monitoring of GM soybean and rice.",
  "abstract_shown": true,
  "affiliations": [
    "Environment Science & Biotechnology, Jeonju University, Jeonju, Republic of Korea.",
    "Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju, Korea."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Areum Jeong",
      "orcid": "0009-0001-2052-5416"
    },
    {
      "affs": [
        1
      ],
      "name": "Sung-Dug Oh",
      "orcid": "0000-0001-8574-6773"
    },
    {
      "affs": [
        0
      ],
      "name": "Sara Noh",
      "orcid": "0009-0001-6597-6034"
    },
    {
      "affs": [
        0
      ],
      "name": "Hong Min"
    },
    {
      "affs": [
        1
      ],
      "name": "Jong Chan Park",
      "orcid": "0000-0002-0799-7742"
    },
    {
      "affs": [
        1
      ],
      "name": "Dohyeon Kwon",
      "orcid": "0009-0008-4234-9880"
    },
    {
      "affs": [
        0
      ],
      "name": "Bumkyu Lee",
      "orcid": "0000-0001-9661-9606"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7ztp6qvy17hge.bib",
  "cited_by": 0,
  "doi": "10.1080/21645698.2026.2744012",
  "fulltext_url": "https://europepmc.org/articles/PMC13652058",
  "id": "7ztp6qvy17hge",
  "issns": [
    "2164-5698",
    "2164-5701"
  ],
  "landing_url": "https://europepmc.org/article/MED/42850589",
  "language": "eng",
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1110|Genetically Modified Organisms Research"
    ]
  ],
  "openalex": "W7220954541",
  "pdf_url": "https://europepmc.org/articles/PMC13652058?pdf=render",
  "pmcid": "PMC13652058",
  "pmid": "42850589",
  "published": "2026-10-08",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/7ztp6qvy17hge.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:02Z",
      "native_id": "MED:42850589",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42850589"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1110|Genetically Modified Organisms Research",
      "subfield": "plant-biology"
    }
  ],
  "title": "Rapid recombinase polymerase amplification-based detection of the bar gene for environmental monitoring of genetically modified soybean and rice.",
  "title_text": "Rapid recombinase polymerase amplification-based detection of the bar gene for environmental monitoring of genetically modified soybean and rice.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:02Z",
  "url": "https://research.draconitic.com/paper/7ztp6qvy17hge",
  "venue": "GM crops & food",
  "versions": []
}