{
  "abstract": "A fluorene-conjugated diazirine is described, for which two-photon activation is demonstrated for the first time.Diazirines are versatile carbene precursors that are extensively used in biological target identification experiments. However, their photo-activation wavelength (ca. 365 nm) precludes their use in living organisms. Here we show that a reconceptualization of the diazirine hybridization state leads to conjugation of the diazirine motif to longer-wavelength chromophores. In a model diazirine–fluorene conjugate, we are able to achieve direct activation (and subsequent C–H insertion) with >450 nm light for the first time. Two-photon activation using near-IR light is also achieved, suggesting the possibility to prepare new diazirine probes for conducting target identification experiments in deep tissue.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 13,
  "authors": [
    {
      "name": "Lorenzo Michelini"
    },
    {
      "name": "Tanya Slaney"
    },
    {
      "name": "Seerat Virk"
    },
    {
      "name": "Estefanía Rafic"
    },
    {
      "name": "L. Charlie Qie"
    },
    {
      "name": "Klara Corejova"
    },
    {
      "name": "Mathieu Lepage"
    },
    {
      "name": "Stefania Musolino"
    },
    {
      "name": "Allen Oliver"
    },
    {
      "name": "Roberto Etchenique"
    },
    {
      "name": "W. David Hong"
    },
    {
      "name": "Gino Dilabio"
    },
    {
      "name": "Jeremy Wulff"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/7ztt7th0abesq.bib",
  "cited_by": 10,
  "doi": "10.1039/d4sc06427e",
  "fulltext_url": null,
  "id": "7ztt7th0abesq",
  "issns": [
    "2041-6520",
    "2041-6539"
  ],
  "landing_url": "https://hal.science/hal-04939066v1",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.chim"
    ],
    [
      "hal",
      "1.chim.orga"
    ],
    [
      "hal",
      "1.chim.theo"
    ],
    [
      "openalex",
      "1606|Chemical Reactions and Mechanisms"
    ]
  ],
  "openalex": "W4404921514",
  "pdf_url": "https://hal.science/hal-04939066/document",
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-03",
  "publisher": "The Royal Society of Chemistry",
  "ris": "https://research.draconitic.com/paper/7ztt7th0abesq.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T12:20:52Z",
      "native_id": "hal-04939066",
      "source": "hal",
      "url": "https://hal.science/hal-04939066v1"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "hal:1.chim.orga",
      "subfield": "organic"
    },
    {
      "field": "chemistry",
      "origin": "hal:1.chim.theo",
      "subfield": "theoretical"
    }
  ],
  "title": "A diazirine's central carbon is sp²-hybridized, facilitating conjugation to dye molecules",
  "title_text": "A diazirine's central carbon is sp²-hybridized, facilitating conjugation to dye molecules",
  "type": "peer-reviewed",
  "updated_at": "2026-11-05T12:20:12Z",
  "url": "https://research.draconitic.com/paper/7ztt7th0abesq",
  "venue": "Chemical Science",
  "versions": []
}