{
  "abstract": "The intersection of actin filaments with the plasma membrane (PM) in plant cells involves lipid-protein interactions by the Arabidopsis class VIII myosin ATM2 that are currently not understood. Using pollen tube cells as a model, we describe how ATM2 enables actin-PM contacts by modulating membrane lipid nano-organization. ATM2 bound anionic PM lipids, including phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P₂), with no apparent preference for any particular lipid species. Overexpression of ATM2 unexpectedly stabilized actin dynamics and altered pollen tube morphology. Actin dynamics remained unaltered upon overexpression of a lipid-binding-defective ATM2 variant. ATM2 effects on actin dynamics required PtdIns(4,5)P₂, as artificial PtdIns(4,5)P₂ depletion in pollen tubes abolished the effects of full-length ATM2 on actin dynamics despite its retained PM association. ATM2 colocalized and interacted with the PI4P 5-kinase PIP5K2, which resides at actin-PM contacts and forms PtdIns(4,5)P₂ nanodomains facilitating rho-of-plants-dependent actin stabilization. Upon ATM2 overexpression, a fluorescent PtdIns(4,5)P₂ biosensor decorated an extended PM region in vivo, indicating a promoting effect of ATM2 on PtdIns(4,5)P₂ abundance. The catalytic activity of purified recombinant PIP5K2 protein was enhanced upon coincubation with a purified carboxy-terminal ATM2 fragment in vitro, suggesting that ATM2 contributes to the intrinsic regulation of PtdIns(4,5)P₂ formation. Actin dynamics are thus stabilized at the actin-PM interface by ATM2 locally promoting the formation of PtdIns(4,5)P₂ nanodomains, thereby self-reinforcing ATM2 recruitment while also facilitating rho of plants activation to stabilize membrane-proximal actin filaments.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Plant Biochemistry, Institute of Biochemistry and Biotechnology, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.",
    "Department of Biophysical Chemistry, Institute of Chemistry, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.",
    "Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic.",
    "Department of Plant Biochemistry, Institute of Biochemistry and Biotechnology, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany. ingo.heilmann@biochemtech.uni-halle.de."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Vera C Wagner"
    },
    {
      "affs": [
        0
      ],
      "name": "Marta Fratini",
      "orcid": "0000-0003-0934-3854"
    },
    {
      "affs": [
        0
      ],
      "name": "Johanna Uhlenberg"
    },
    {
      "affs": [
        0
      ],
      "name": "Alexandra Schutkowski"
    },
    {
      "affs": [
        0
      ],
      "name": "Gottfried Hamm"
    },
    {
      "affs": [
        0
      ],
      "name": "Christoph Kastner"
    },
    {
      "affs": [
        1
      ],
      "name": "Kirsten Bacia"
    },
    {
      "affs": [
        2
      ],
      "name": "Kateřina Schwarzerová"
    },
    {
      "affs": [
        3
      ],
      "name": "Ingo Heilmann",
      "orcid": "0000-0002-2324-1849"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/80cy56zm313x2.bib",
  "cited_by": 0,
  "doi": "10.1038/s41477-026-02427-0",
  "fulltext_url": "https://europepmc.org/articles/PMC13680279",
  "id": "80cy56zm313x2",
  "issns": [
    "2055-0278"
  ],
  "landing_url": "https://europepmc.org/article/MED/42888236",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Plant Reproductive Biology"
    ]
  ],
  "openalex": "W7222578149",
  "pdf_url": "https://europepmc.org/articles/PMC13680279?pdf=render",
  "pmcid": "PMC13680279",
  "pmid": "42888236",
  "published": "2026-10-16",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/80cy56zm313x2.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:46Z",
      "native_id": "MED:42888236",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42888236"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Plant Reproductive Biology",
      "subfield": "molecular-biology"
    }
  ],
  "title": "The class VIII myosin ATM2 stabilizes actin-plasma membrane contacts in tobacco pollen tubes by locally promoting PtdIns(4,5)P₂ formation.",
  "title_text": "The class VIII myosin ATM2 stabilizes actin-plasma membrane contacts in tobacco pollen tubes by locally promoting PtdIns(4,5)P₂ formation.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:46Z",
  "url": "https://research.draconitic.com/paper/80cy56zm313x2",
  "venue": "Nature plants",
  "versions": []
}