{
  "abstract": "Cells must prevent incompletely spliced transcripts from entering the cytoplasm, yet how splicing status is recognized and converted into selective nuclear retention remains unclear. Here, we identify a network of RNA-binding proteins that retain pre-mRNPs within nuclear speckles. Proteomic analysis of splicing intermediates accumulated in nuclear speckles identified a set of retention factors including BCLAF1, THRAP3 and SRSF7 that anchor pre-mRNPs to the SON scaffold and are required for pre-mRNP retention in speckles. Distinct RNA-binding patterns and transcript-specific retention factor requirements suggest that multiple mRNP features tune retention combinatorially. Super-resolution imaging revealed that the SON and SRRM2 speckle scaffolds form interdigitated networks with RNA enriched along networks and within interstitial speckle lumens. This architecture remodels in response to pre-mRNP load and is disrupted upon depletion of retention factors. Together, these findings define a pre-mRNP quality-control pathway that links splicing state to nuclear retention and the dynamic organization of nuclear speckles.",
  "abstract_shown": true,
  "affiliations": [
    "Biozentrum, University of Basel, Basel, Switzerland."
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 14,
  "authors": [
    {
      "name": "K. Doerner"
    },
    {
      "name": "K. Kurth"
    },
    {
      "name": "N. Beuret"
    },
    {
      "name": "N. Beer"
    },
    {
      "name": "M. K. Jones"
    },
    {
      "name": "K. Davey"
    },
    {
      "name": "L. Stadelmann"
    },
    {
      "name": "D. Huegli"
    },
    {
      "name": "A. Ferrand"
    },
    {
      "name": "G. Basile"
    },
    {
      "name": "S. Luescher"
    },
    {
      "name": "J. Ule"
    },
    {
      "name": "F. Voigt"
    },
    {
      "affs": [
        0
      ],
      "name": "M. Hondele"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/80jfzwpstmyhn.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.28.754972",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.28.754972v1.full",
  "id": "80jfzwpstmyhn",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.28.754972v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "cell biology"
    ],
    [
      "openalex",
      "1312|RNA Research and Splicing"
    ]
  ],
  "openalex": "W7214759770",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.28.754972v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-28",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/80jfzwpstmyhn.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:25:04Z",
      "native_id": "10.64898/2026.09.28.754972",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.28.754972v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:cell biology",
      "subfield": "cell-biology"
    }
  ],
  "title": "A combinatorial pre-mRNP retention factor network couples nuclear speckle architecture to splicing quality control",
  "title_text": "A combinatorial pre-mRNP retention factor network couples nuclear speckle architecture to splicing quality control",
  "type": "preprint",
  "updated_at": "2026-10-30T12:25:04Z",
  "url": "https://research.draconitic.com/paper/80jfzwpstmyhn",
  "venue": "bioRxiv",
  "versions": []
}