{
  "abstract": "The visualisation of large biological samples in cryo-electron tomography requires both few-nanometre resolution and a wide, ideally >10 micrometrre, field of view. For a single image, increasing magnification trades increased resolution for a smaller field of view. Montaging, where high-magnification images are stitched together to increase field of view, addresses the trade-off but is dose-inefficient with round beams that are standard in transmission electron microscopy (TEM), since this illumination shape does not tessellate. Our workflow greatly simplifies montaging schemes for dose-sensitive specimens, allowing large montages of any shape - from individual cells or other features of interest up to whole lamellae - to be collected at high resolution without compromising electron dose efficiency. We demonstrate the workflow by acquiring montage tomograms of whole yeast (Saccharomyces cerevisiae) cells in cryo-FIB lamellae and of human neurons.",
  "abstract_shown": true,
  "affiliations": [
    "Ian Holmes Imaging Centre, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Victoria, Australia"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "name": "H. G. Brown"
    },
    {
      "name": "S. Valimehr"
    },
    {
      "name": "D. Styczynski"
    },
    {
      "name": "M. Mudaliyar"
    },
    {
      "name": "S. S. Kundapura"
    },
    {
      "name": "M. D. Johnson"
    },
    {
      "name": "D. Ghosal"
    },
    {
      "affs": [
        0
      ],
      "name": "E. Hanssen"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/80m850r76rg98.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.27.753993",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.27.753993v1.full",
  "id": "80m850r76rg98",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.27.753993v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "biophysics"
    ],
    [
      "openalex",
      "1315|Advanced Electron Microscopy Techniques and Applications"
    ]
  ],
  "openalex": "W7214616685",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.27.753993v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-27",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/80m850r76rg98.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:24:44Z",
      "native_id": "10.64898/2026.09.27.753993",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.27.753993v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "biorxiv:biophysics",
      "subfield": "biophysics"
    }
  ],
  "title": "Whole cryo-FIB lamella acquisition at high magnification with rectangular beam montage cryo-electron tomography",
  "title_text": "Whole cryo-FIB lamella acquisition at high magnification with rectangular beam montage cryo-electron tomography",
  "type": "preprint",
  "updated_at": "2026-10-30T12:24:44Z",
  "url": "https://research.draconitic.com/paper/80m850r76rg98",
  "venue": "bioRxiv",
  "versions": []
}